ReviewPolymers2025
Methods for Determining the High Molecular Weight of Hyaluronic Acid: A Review.
Review in Polymers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- A systematic review of structure-activity relationships in bacterial levan: physicochemical mediation and context-dependent functionality.RSC advances · 2026Review
- Hybrid 3D Printing of Interfacial Polyelectrolyte Complex Formed between Hyaluronic Acid and Poly-L-lysine Hydrogels.ACS omega · 2026Article
- Fabrication of Size-Controlled Carbon Dots with Biofilm-Disrupting Activity for Antibacterial Applications.International journal of molecular sciences · 2026Article
- Microbial Hyaluronidases: From Obscure Virulence Factors to Promising Therapeutic Targets.Biomolecules · 2026Review
- Hyaluronic Acid-Based Gels and Biomaterial Systems for Oral Wound Healing: Design and Clinical Translation.Gels (Basel, Switzerland) · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronic acid (HA) is a key extracellular biopolymer whose molecular weight (MW) critically determines its physicochemical behaviour and biological functionality. Accurate MW characterization is therefore essential for applications ranging from tissue engineering to medical and cosmetic formulations. Although previous reviews have addressed general aspects of HA, they have only briefly summarized analytical approaches for MW determination. In contrast, the present review provides the first dedicated and comparative analysis of the three most widely used and practically relevant techniques-agarose gel electrophoresis, size-exclusion chromatography coupled with HPLC (SEC-HPLC) and intrinsic viscosity measurements. We examine recent methodological advances, including improved calibration strategies, optimized gel matrices, multi-detector SEC configurations, and updated empirical models for viscometric analysis. Particular emphasis is placed on how these innovations enhance accuracy, reproducibility and applicability across different HA size ranges and sample purities. By critically evaluating the strengths, limitations and sources of analytical bias of each technique, this review offers a consolidated framework to guide researchers and industry professionals in selecting the most appropriate method for reliable MW assessment. Overall, we highlight how methodological refinement is enabling more robust characterization of HA, even in highly polydisperse or complex samples.
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Registered trials
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