Evidence map›Paper›PMID 41469998›Full record

ArticleBMC veterinary research2025

Monoclonal antibody preparation and linear epitope identification for the ASFV A151R protein.

Zhibo Liu, Jianyi You, WenBo Zhang, Chenyang Gao, Shaohong Huang, Lang Gong, Xiongnan Chen, Guihong Zhang, Yiwei Huang, Zhao Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhibo Liu *Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Jianyi You *Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
WenBo ZhangKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Chenyang GaoKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Shaohong HuangKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Lang GongKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Xiongnan ChenKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guihong ZhangKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Yiwei HuangDaguang Food Research Institute, Jiangmen, China. wzms2009@163.com.
Zhao HuangKey Laboratory of Zoonosis Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China. yingwenmulu@163.com.

Funding

China Agriculture Research System of MOF and MARA CARS-35Guangzhou Basic and Applied Basic Research Foundation 202201010490National Key Research and Development Program of China 2021YFD1800100The 77th Batch of General support from the China Postdoctoral Foundation 2025M773025
6 · The paper itself

Abstract

African Swine fever (ASF) is an acute, hemorrhagic infectious disease caused by the African swine fever virus (ASFV), posing a major threat to the global pig farming industry. ASFV is a complex DNA virus. Its non-structural protein, A151R, is a thioredoxin crucial for the replication and morphogenesis of ASFV. In this study, a monoclonal antibody (mAb) 3E5D6 against pA151R (A151R Protein) was prepared using cell fusion technology to gain deeper insight into the function of A151R during ASFV infection. Immunolabeling of pA151R with 3E5D6 mAb revealed its involvement in viral factory formation and its characteristic early expression during infection. However, the antibody exhibited no detectable neutralizing activity. Subsequently, the antigenic epitope

Indexed as

African Swine Fever VirusAntibodies, MonoclonalAntibodies, ViralEpitopesViral Nonstructural ProteinsAfrican Swine FeverAmino Acid SequenceAnimalsMice, Inbred BALB CSwineAntibodies, MonoclonalAntibodies, ViralEpitopesViral Nonstructural ProteinsASFV pA151RB cell epitopeMonoclonal antibody

Identifiers

PMID41469998
PMCPMC12859835

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