Evidence map›Paper›PMID 41469844›Full record

ArticleBMC genomics2025

Comparative genomics suggests extensive antithrombotic gene expansion in Haemadipsa yanyuanensis.

Yiquan Lin, Fang Zhao, Sijia Fan, Dezhi Yang, Xiangrong Tong, Lizhou Tang, Dejun Kong, Gonghua Lin, Zichao Liu

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yiquan LinSchool of Medicine, Kunming University, Kunming, 650214, China.
Fang ZhaoSchool of Life Sciences, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, Jinggangshan University, Ji'an, 343009, China.
Sijia FanEngineering Research Center for Exploitation and Utilization of Leech Resources in Universities of Yunnan Province, School of Agriculture and Life Sciences, Kunming University, Kunming, 650214, China.
Dezhi YangEngineering Research Center for Exploitation and Utilization of Leech Resources in Universities of Yunnan Province, School of Agriculture and Life Sciences, Kunming University, Kunming, 650214, China.
Xiangrong TongEngineering Research Center for Exploitation and Utilization of Leech Resources in Universities of Yunnan Province, School of Agriculture and Life Sciences, Kunming University, Kunming, 650214, China.
Lizhou TangCollege of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China.
Dejun KongSchool of Ecology and Environmental Science, Yunnan University, Kunming, 650500, China. kongdj@ynu.edu.cn.
Gonghua LinSchool of Life Sciences, Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity, Jinggangshan University, Ji'an, 343009, China. lingonghua@163.com.
Zichao LiuSchool of Medicine, Kunming University, Kunming, 650214, China. abclzc@aliyun.com.

Funding

2023 Frontier Research Team Program of Kunming University, the Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity 2023SSY02111Joint Special Project for Basic Research of Local Universities in Yunnan Province 202101BA070001-164Joint Special Project for Basic Research of Local Universities in Yunnan Province 202301BA070001-105National Key R&D Program of China 2023YFF1305000National Natural Science Foundation of China 32260132National Natural Science Foundation of China 82260742Special Project of Science-Technology Talent and Platform in Yunnan Province 202105AC160042Yunnan International Joint Laboratory with South and Southeast Asia for the Integrated Development of Animal-Derived Anti-Thrombosis Chinese Medicine 202503AP140025Yunnan Provincial University Serving Key Industry Science and Technology Special Project FWCY-ZD2024009Yunnan Ten Thousand Talents Program for Young Top Notch Talents YNWR-QNBJ-2020-122
6 · The paper itself

Abstract

Haemadipsa yanyuanensis Liu & Song, 1977, is a terrestrial blood-feeding leech whose antithrombotic repertoire remains insufficiently characterized. We present its first chromosome-level genome assembly (165.32 Mb across nine chromosomes; BUSCO: 97.6%) using Nanopore long reads, Hi-C scaffolding, and RNA-seq data. We identified 193 putative antithrombotic genes in 15 putative families, representing a 2.2- to 2.7-fold increase in gene number but reduced family diversity compared with aquatic medicinal leeches, including Hirudo nipponia Whitman, 1886, Hirudinaria manillensis Lesson, 1842, and Hirudo medicinalis Linnaeus, 1758. Notably, the putative bdellin, LDTI, and LCI gene families exhibited marked lineage-specific expansions, ranging from 8.7- to 25-fold relative to those of aquatic leeches. In addition, the putative progranulin gene displayed a distinctive structural organization characterized by 122 cysteine residues and nine tandem repeats. Transcriptomic profiling revealed elevated expression of four expanded putative families, suggesting potential roles in terrestrial blood-feeding adaptation. This chromosome-scale genome provides a valuable resource for investigating anticoagulation mechanisms and may inform the development of next-generation antithrombotic therapeutics.

Indexed as

Fibrinolytic AgentsGenomicsLeechesAnimalsGene Expression ProfilingGenomePhylogenyTranscriptomeFibrinolytic AgentsAntithrombotic genesChromosome-scale genomeGene family evolutionHaemadipsa yanyuanensisTerrestrial leech

Identifiers

PMID41469844
PMCPMC12866057

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.