ArticleNPJ science of food2025
Endonuclease V activated Pyrococcus furiosus Argonaute for the detection of food contaminated bacteria.
Article in NPJ science of food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- PfAgo-Enhanced LAMP Assay for Rapid and Specific Detection ofFoods (Basel, Switzerland) · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
Pyrococcus furiosus Argonaute (PfAgo) is a novel programmable nuclease that has been used in nucleic acid detection due to its excellent performance. Traditional PfAgo based detection methods relies on the input of exogenous guide DNA (gDNA), which restricted its flexibility and universality in application. Here, we designed primers with deoxy inosine base which can be recognized and cleaved by endonuclease V, following turn into gDNA to activate PfAgo for target gene detection. Therefore, an Endo V activated PfAgo based nucleic acid detection (VPN) method was developed. The detection limit of this method was 0.04 ng/μL DNA. Moreover, the method was successfully applied to the detection of food contaminated bacteria. This approach provided a universal and power tool for the detection of nucleic acid-containing organisms such as pathogens, viruses, and tumor cells.
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Registered trials
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