Evidence map›Paper›PMID 41468958›Full record

ArticleJournal of advanced research2026

Hypoxia inhibits intervertebral disc degeneration by maintaining autophagy circadian rhythm via the HIF1α-PER2-mTOR pathway.

Guang-Cheng Yuan, Qi-Chen Zhang, Yu-Xiang Ge, Heng-Jie Zeng, Tai-Wei Zhang, Wang Ding, Zhi-Rui Dong, Yu-Kai Huang, Jian Dong, Nong Chen and 1 more

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In one paragraph

Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Guang-Cheng YuanDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Qi-Chen ZhangDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China; Department of Orthopedic Surgery, Shanghai Xuhui District Central Hospital, Fudan University, Shanghai 200031, China.
Yu-Xiang GeDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Heng-Jie ZengDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Tai-Wei ZhangDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Wang DingDepartment of Orthopedic Surgery, Minhang Hospital, Fudan University, Shanghai 201100, China.
Zhi-Rui DongDepartment of Orthopedic Surgery, Jinshan Hospital of Fudan University, Shanghai 201508, China.
Yu-Kai HuangDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Jian DongDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address: dong.jian@zs-hospital.sh.cn.
Nong ChenDepartment of Orhtopedics, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, China. Electronic address: chennong1975@126.com.
Li-Bo JiangDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address: jiang.libo@zs-hospital.sh.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionDisruption of the circadian rhythm (CR) and autophagy in intervertebral discs contributes to intervertebral disc degeneration (IDD) progression. However, the circadian regulation of autophagy requires further investigation.

objectivesWe observed the expression of circadian proteins and autophagic markers of nucleus pulposus (NP) cells followed a diurnal rhythmic pattern in vivo and in vitro.

methodsNP tissues were collected from light/dark cycle-shifted rats and IDD patients of varying severity. CR and ECM-related proteins were analyzed by immunohistochemistry and western blotting. Primary rat NP cells were treated with hypoxia or CoCl

resultsThe silencing of clock gene PER2 disrupted the rhythmic expression of autophagic markers, by contrast PER2 overexpression inhibited mTOR pathway and enhanced autophagic levels. Co-IP analysis demonstrated the PER2-mTOR interaction, linking CR and autophagy rhythm. The inflammatory stimulator dampened the CR and autophagy rhythm, however intermittent hypoxia and cobalt chloride (CoCl

conclusionOur study demonstrates that hypoxia maintains the intrinsic CR and autophagy rhythm through the HIF-1α/PER2/mTOR pathway to prevent IDD.

Indexed as

AutophagyCircadian RhythmHypoxiaHypoxia-Inducible Factor 1, alpha SubunitIntervertebral Disc DegenerationPeriod Circadian ProteinsTOR Serine-Threonine KinasesAnimalsCell HypoxiaCobaltFemaleHumansMaleNucleus PulposusRatsRats, Sprague-DawleyCobaltcobaltous chlorideHif1a protein, ratHypoxia-Inducible Factor 1, alpha SubunitPER2 protein, humanPeriod Circadian ProteinsTOR Serine-Threonine KinasesAutophagyCircadian rhythmHIF1αHypoxiaIntervertebral disc degeneration

Identifiers

PMID41468958
PMCPMC13669551

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.