ArticlemSystems2026
Elucidation of population-based bacterial adaptation to antimicrobial treatment by single-cell sequencing analysis of the gut microbiome of a hospital patient.
Lianwei Ye, Yuchen Wu, Jiubiao Guo, Hanyu Wang, Jing Cai, Kaichao Chen, Ning Dong, Jiale Yu, Shan Chao, Hongwei Zhou and 3 more
Abstract read
In one paragraphArticle in mSystems, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
13 authors.
Lianwei Ye *Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.ORCID 0000-0002-2301-9930 Yuchen Wu *Department of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
Jiubiao Guo *Clinical Research Center, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.
Hanyu WangDepartment of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.ORCID 0009-0005-5127-5842 Jing CaiNeuroscience Intensive Care Unit, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
Kaichao ChenDepartment of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
Ning DongSchool of Public Health, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.ORCID 0000-0001-6515-4201 Jiale YuMobiDrop (Zhejiang) Co., Ltd, Shanghai, China.
Shan ChaoMobiDrop (Zhejiang) Co., Ltd, Shanghai, China.
Hongwei ZhouDepartment of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.ORCID 0000-0002-9478-4023 Gongxiang ChenDepartment of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.ORCID 0000-0001-5730-6797 Sheng ChenState Key Laboratory of Chemical Biology and Drug Discovery and the Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, Hong Kong.ORCID 0000-0003-3526-7808 Rong ZhangDepartment of Clinical Laboratory, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.ORCID 0000-0002-2174-7985 Funding
general research fund of research grant councile of the Government of Hong Hong SAR 11100321 11100922National Key Research and Development Program of China No. 2022YFD1800400National Natural Science Foundation of China 82272392Theme-base research scheme T11-104/22-R
6 · The paper itselfAbstract
In this study, we used single-cell sequencing to analyze the gut microbiome of an adult male patient with acute cerebral hemorrhage undergoing antibiotic treatment. We identified 92 bacterial species, including 23 Firmicutes and one archaeon from Methanobacteriota, along with 69 unclassified strains. Single-cell sequencing effectively detected bacteria carrying antibiotic resistance genes (ARGs), particularly in unclassified species, and traced the evolution of these genes across diverse bacterial taxa. Notably, the
Indexed as
Anti-Bacterial AgentsBacteriaGastrointestinal MicrobiomeAdaptation, PhysiologicalDrug Resistance, BacterialGene Transfer, HorizontalHumansMaleSingle-Cell AnalysisAnti-Bacterial Agentsantibiotic resistance genes (ARGs)gut microbiomehorizontal gene transfer (HGT)Klebsiella pneumoniaesingle-cell sequencing
Identifiers
PMID41468549
PMCPMC12911351
What OpenQuestion holds
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LicenceCC BY
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