ArticleCurrent protocols2026
Optimized Laboratory Maintenance and Functional Testing of Chara braunii.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Circadian Oscillations in Branchlet Angle and Irradiance-Dependent Acclimation inPlant direct · 2026Article
- Optimized Laboratory Maintenance and Functional Testing of Chara braunii.Current protocols · 2026Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chara braunii is an emerging model species for studying plant evolution and development. Various members of the Chara genus have been used for pioneering studies of electrophysiology, cytoplasmic streaming, and cell biology. However, many studies have been limited by challenges, such as overgrowth with epiphytes or non-continuous growth throughout the year. Here, we present protocols for the cultivation, germination, and in vivo staining of C. braunii NIES-1604 to overcome these limitations. We constructed the custom-made cultivation chamber equipped with the illumination unit that allows for the precise simulation of natural conditions and continuous cultivation of C. braunii NIES-1604 throughout the year. By determining the optimal stratification period for C. braunii NIES-1604 oospores, we achieved a germination rate of 26%. Further, we present fluorescence-based in vivo staining protocols for routine in vivo staining of cellular structures including the plasma membrane, cell wall, and nuclei. Altogether, our affordable method of controlled cultivation of C. braunii NIES-1604 and other procedures serve as a valuable resource for establishing Chara cultures in laboratory settings and advancing its use as a model organism. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Construction of a cultivation chamber for C. braunii NIES-1604 growth Basic Protocol 2: Vegetative propagation of C. braunii NIES-1604 Basic Protocol 3: Germination of C. braunii NIES-1604 oospores Basic Protocol 4: In vivo staining of C. braunii NIES-1604 with fluorescent dyes.
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Registered trials
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