Evidence map›Paper›PMID 41467461›Full record

ArticleThe plant genome2026

A high-recovery, high-density targeted genotyping platform for cranberry.

Shaun J Clare, Ping Zheng, Mandie Driskill, Joseph Kawash, Jeffrey Neyhart, Timothy R Millar, Dorrie Main, James Polashock, Patrick Edger, Nicholi Vorsa and 6 more

Abstract read
In one paragraph

Article in The plant genome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Shaun J ClareUSDA-ARS National Clonal Germplasm Repository, Corvallis, Oregon, USA.ORCID https://orcid.org/0000-0003-2887-3404
Ping ZhengDepartment of Crop and Soil Science and Department of Horticulture, Washington State University, Pullman, Washington, USA.ORCID https://orcid.org/0009-0004-2302-5638
Mandie DriskillUSDA-ARS National Clonal Germplasm Repository, Corvallis, Oregon, USA.
Joseph KawashUSDA-ARS, Chatsworth, New Jersey, USA.ORCID https://orcid.org/0000-0003-4168-1319
Jeffrey NeyhartUSDA-ARS, Chatsworth, New Jersey, USA.ORCID https://orcid.org/0000-0002-1991-5310
Timothy R MillarThe New Zealand Institute for Plant and Food Research Ltd., Lincoln, New Zealand.ORCID https://orcid.org/0000-0002-5142-8811
Dorrie MainDepartment of Crop and Soil Science and Department of Horticulture, Washington State University, Pullman, Washington, USA.ORCID https://orcid.org/0000-0002-1162-2724
James PolashockUSDA-ARS, Chatsworth, New Jersey, USA.ORCID https://orcid.org/0000-0002-7977-2921
Patrick EdgerDepartment of Horticulture, Michigan State University, East Lansing, Michigan, USA.ORCID https://orcid.org/0000-0001-6836-3041
Nicholi VorsaDepartment of Plant Biology, Rutgers University, New Brunswick, New Jersey, USA.ORCID https://orcid.org/0000-0002-7976-3486
Jennifer Johnson-CicaleseDepartment of Plant Biology, Rutgers University, New Brunswick, New Jersey, USA.
Andrew F MauleUSDA-ARS, Vegetable Crops Research Unit, Plant and Agroecosystem Sciences Department, University of Wisconsin, Madison, Wisconsin, USA.ORCID https://orcid.org/0000-0002-3187-1461
Juan ZalapaUSDA-ARS, Vegetable Crops Research Unit, Plant and Agroecosystem Sciences Department, University of Wisconsin, Madison, Wisconsin, USA.ORCID https://orcid.org/0000-0001-8840-3565
Gina M SideliDepartment of Plant Biology, Rutgers University, New Brunswick, New Jersey, USA.ORCID https://orcid.org/0000-0003-1056-1496
Massimo IorizzoDepartment of Horticultural Science, Plants for Human Health Institute, North Carolina State University, Kannapolis, North Carolina, USA.
Nahla BassilUSDA-ARS National Clonal Germplasm Repository, Corvallis, Oregon, USA.ORCID https://orcid.org/0000-0001-8625-2740

Funding

Agricultural Research Service CRIS Project 2072-21000-059-000DNational Institute of Food and Agriculture AFRI VacciniumCAPNational Institute of Food and Agriculture SCRI Award 2019-51181-30015
6 · The paper itself

Abstract

The American cranberry (Vaccinium macrocarpon) is a commercially important berry species native to North America. Cranberry is a popular fruit crop with many known health benefits. Thus far, genotyping-by-sequencing (GBS) was the only high-throughput genotyping technique available in cranberry. While GBS is cost efficient, it has the disadvantage of producing variant calls that cannot be easily transferred between populations and across studies. In this study, we report the development of the first targeted high-throughput genotyping platform for cranberry. The Flex-Seq genotyping technology offers repeatable, high-recovery, and high-density single nucleotide polymorphisms as well as concomitant haplotypes. The cranberry Flex-Seq platform contains 17,502 loci, boasting a recovery rate of 99.8% in a diversity panel of 192 cranberry accessions. The level of incompatible marker inheritance in 22 analyzed parent-offspring trios ranged from 1.1% to 13%, with an average of 5.5%. Nine of the 22 crosses had an excess of incompatible markers, indicating inconsistent parentage that could be investigated further. In quantitative trait loci (QTLs) analyses, Flex-Seq identified a greater number of QTLs compared to previously used GBS as well as higher overall logarithm of odds scores for overlapping QTLs. In addition, construction of locus haplotypes resulted in a substantial improvement in resolving the relatedness of cranberry accessions. This platform will aid in utilizing the untapped potential of wild cranberry, mapping key traits with higher precision, and ensuring robust characterization of accessions. The developed genotyping platform will help in the rapid improvement of cranberry cultivars to benefit breeders, growers, and consumers.

Indexed as

Genotyping TechniquesVaccinium macrocarponGenotypeHaplotypesHigh-Throughput Nucleotide SequencingPolymorphism, Single NucleotideQuantitative Trait Loci

Identifiers

PMID41467461
PMCPMC12750414

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.