Evidence map›Paper›PMID 41466030›Full record

ArticleScientific reports2025

First molecular confirmation of Lasiodiplodia theobromae causing grapevine trunk disease in southern Egypt.

Youssuf A Gherbawy, Nabila A Hassany, Eman G A M El-Dawy

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Youssuf A GherbawyDepartment of Botany and Microbiology, Faculty of Science, South Valley University (Qena University), Qena, 83523, Egypt.
Nabila A HassanyDepartment of Botany and Microbiology, Faculty of Science, South Valley University (Qena University), Qena, 83523, Egypt.
Eman G A M El-DawyDepartment of Botany and Microbiology, Faculty of Science, South Valley University (Qena University), Qena, 83523, Egypt. emymycology@yahoo.com.ORCID 0000-0003-1756-1018

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Grapevine trunk diseases constitute a significant phytopathological concern in Egyptian viticulture, with ongoing debates regarding their origin and transmission dynamics. These complexities are attributed to the heterogeneous manifestation of symptoms and the involvement of multiple wood-associated pathogens, both suspected and confirmed. This study investigates the mycological aspects of grapevine trunk diseases, focusing on Lasiodiplodia theobromae as a causal agent. The pathogen was associated with vascular cankers, dark brown trunk discoloration, pycnidia formation on necrotic tissues, and grapevine dieback. Identification of L. theobromae was achieved through morphological characteristics and molecular analysis targeting the β-tubulin gene and Internal Transcribed Spacer (ITS) region. Pathogenicity tests were conducted by inoculating detached canes, leaves, petioles, and entire branches with mycelial plugs of L. theobromae. The resulting symptoms closely resembled those observed in naturally infected grapevines in the field. The pathogen was then re-isolated and identified, confirming Koch's postulates. A disease index (DI) ranging from 60 to 100% provided strong evidence of the high pathogenic potential of L. theobromae under experimental conditions.

Indexed as

AscomycotaPlant DiseasesVitisEgyptPhylogenyPlant LeavesTubulinTubulinEgyptian vineyardsLasiodiplodia theobromaePathogenicity testPhylogeny

Identifiers

PMID41466030
PMCPMC12753789

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