Evidence map›Paper›PMID 41465737›Full record

ArticleLife (Basel, Switzerland)2025

In Vivo Micro-Computed Tomography for Evaluation of Osteogenic Capability of Dental Pulp Stem Cells Under the Influence of Extracellular Vesicles on Alloplastic and Xenogeneic Bone Scaffolds in Rodent Intrabony Defect Model.

Marius Heitzer, Philipp Winnand, Mark Ooms, Elizabeth R Balmayor, Frank Hildebrand, Christian Apel, Zuzanna Magnuska, Fabian Kiessling, Frank Hölzle, Ali Modabber

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Marius HeitzerDepartment of Oral- and Maxillofacial Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.ORCID 0000-0002-9205-6065
Philipp WinnandDepartment of Oral- and Maxillofacial Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.ORCID 0000-0001-8315-7684
Mark OomsDepartment of Oral- and Maxillofacial Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.ORCID 0000-0002-9785-9502
Elizabeth R BalmayorDepartment of Orthopedics, Trauma and Reconstructive Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.ORCID 0000-0002-0484-4847
Frank HildebrandDepartment of Orthopedics, Trauma and Reconstructive Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.
Christian ApelDepartment of Biohybrid & Medical Textiles, Institute of Applied Medical Engineering, Faculty of Medicine, RWTH Aachen University, Forckenbeckstraße 55, 52074 Aachen, Germany.
Zuzanna MagnuskaInstitute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Forckenbeckstraße 55, 52074 Aachen, Germany.
Fabian KiesslingInstitute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Forckenbeckstraße 55, 52074 Aachen, Germany.ORCID 0000-0002-7341-0399
Frank HölzleDepartment of Oral- and Maxillofacial Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.
Ali ModabberDepartment of Oral- and Maxillofacial Surgery, RWTH Aachen University Hospital, Pauwelsstraße 30, 52074 Aachen, Germany.ORCID 0000-0003-2160-4527

Funding

Interdisciplinary Centre for Clinical Research within the faculty of Medicine at the RWTH Aachen University (OC1-8)
6 · The paper itself

Abstract

Regeneration of jawbone defects poses major challenges. The combination of dental pulp stem cells (DPSCs) or DPSC-derived extracellular vesicles (EVs) with bone substitute materials shows promising potential for bone tissue engineering in vitro. This study evaluated the in vivo bone regeneration potential of DPSCs and EVs with bone graft substitutes in a novel intrabony defect model. DPSCs were isolated from 35 male Sprague-Dawley rat incisors, and EVs were collected from the cell culture medium. DPSCs were seeded onto alloplastic and xenogeneic bone graft materials and implanted into bone defects. Control groups received bone substitutes without DPSCs or EVs. Micro-computed tomography (µCT) was performed at 12 and 24 weeks post-implantation to assess bone volume (BV), bone density (BD), trabecular thickness (Tr.Th), bone growth rate (BGR), and bone-to-mineral ratio (BMR). Both graft types increased BV and BD, with no significant differences between them. Tr.Th increased across all treatments after 24 weeks, indicating ongoing bone remodeling. Notably, xenogeneic grafts combined with DPSCs and EVs significantly improved BGR (

Indexed as

bone regenerationextracellular vesiclesosteogenesisstem cells

Identifiers

PMID41465737
PMCPMC12734100

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.