Assessment of miRNA 106a-5p and 375-3p Expression in the Context of the Wnt/β-Catenin Pathway-Comparison of Prostate Adenocarcinoma and Benign Prostatic Hyperplasia Tissues.

Magdalena Smereczańska, Natalia Domian, Grzegorz Młynarczyk, Irena Kasacka

Abstract readComparative Study

In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Magdalena SmereczańskaDepartment of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.ORCID 0000-0002-3398-366X

Natalia DomianDepartment of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.

Grzegorz MłynarczykDepartment of Urology, Medical University of Bialystok, M. Skłodowskiej-Curie 24A Street, 15-276 Bialystok, Poland.ORCID 0000-0002-1453-8501

Irena KasackaDepartment of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.

Funding

Uniwersytet Medyczny w Białymstoku B.SUB.25.367

6 · The paper itself

Abstract

Prostate adenocarcinoma is mainly diagnosed based on serum PSA levels, but elevated PSA levels can also be caused by BPH, which weakens its specificity. Recent scientific studies have demonstrated that specific microRNAs regulate cancer cell proliferation by modulating the Wnt/β-catenin pathway. To date, no published literature has provided a comprehensive assessment of the interactions between miR-106a-5p and miR-375-3p and components of the Wnt/β-catenin pathway in prostate cancer. Therefore, the aim of the present study was to perform a pilot evaluation of the expression of miRNAs 106a-5p and 375-3p, as well as β-catenin, Fzd8, Wnt5a, and cyclin D1 in prostate adenocarcinoma compared with BPH. The study material consisted of samples collected from 30 patients with prostate cancer and 30 with BPH. Protein expression was analyzed using IHC and qRT-PCR methods, while miRNA levels were quantified by dPCR. Our study results revealed lower immunoreactivity and expression of genes encoding β-catenin, Fzd8, Wnt5a, and cyclin D1 and significantly higher fluorescence intensity of miRNA 106a-5p and 375-3p with prostate adenocarcinoma compared to BPH. These parallel alterations in miRNA expression and Wnt/β-catenin-related components reflect disease-specific expression patterns and warrant further investigation in larger cohorts to determine their potential utility as diagnostic biomarkers in prostate diseases.

Indexed as

AdenocarcinomaMicroRNAsProstatic HyperplasiaProstatic NeoplasmsWnt Signaling PathwayAgedbeta CateninCyclin D1Gene Expression Regulation, NeoplasticHumansMaleMiddle AgedReceptors, Cell SurfaceWnt-5a Proteinbeta CateninCCND1 protein, humanCyclin D1Fzd8 protein, humanMicroRNAsMIRN106 microRNA, humanMIRN375 microRNA, humanReceptors, Cell SurfaceWnt-5a ProteinWNT5A protein, humancyclin D1Fzd8miRNAsprostate adenocarcinomaWnt5aβ-catenin

Identifiers

PMID41465498

DOI10.3390/ijms262412073

PMCPMC12733049

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.