Evidence map›Paper›PMID 41465361›Full record

ArticleInternational journal of molecular sciences2025

Effects of Topography and Extracellular Matrix Composition on Focal Adhesion Patterning in Human Corneal Fibroblasts.

Divya Subramanian, Nathaniel S Tjahjono, Tarik Z Shihabeddin, Satweka Nammi, Miguel Miron-Mendoza, Victor D Varner, W Matthew Petroll, David W Schmidtke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Divya SubramanianDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
Nathaniel S TjahjonoDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.ORCID 0000-0003-2485-6498
Tarik Z ShihabeddinDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
Satweka NammiDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
Miguel Miron-MendozaDepartment of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Victor D VarnerDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.
W Matthew PetrollDepartment of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.ORCID 0000-0003-0795-4539
David W SchmidtkeDepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA.ORCID 0000-0001-6404-318X

Funding

Stem Cell, Organoid and Cell Phenotyping ModuleP30EY030413 · NEI · UT SOUTHWESTERN MEDICAL CENTER · PI W MATTHEW PETROLL · 2019 to 2026
$5.9M
International Foundation for Ethical Research IFERNEI NIH HHS P30 EY030413NIH HHS 1P30EY030413-24NIH HHS 4R01EY030190-24Prevent Blindness Challenge Grant
6 · The paper itself

Abstract

Corneal fibroblasts adhere to the extracellular matrix via integrin-containing focal adhesions (FAs). Although topographical cues are known to influence FA patterning in corneal fibroblasts, it is unclear how ECM composition, biophysical cues, and specific integrins modulate FA patterning in corneal fibroblasts. In this study, we cultured a human corneal fibroblast cell line (HTKs) on different ECM proteins and micropatterns of aligned collagen fibrils to determine the effects of ECM topography and composition on focal adhesion subcellular patterning. Using confocal imaging, we observed and quantified changes in FA and integrin patterning based on the underlying ECM type. More specifically, the presence of fibrillar topography as compared to monomeric collagen resulted in diminished FA number, area, and length. Using specific integrin blocking antibodies, we also demonstrate that HTKs use different integrin subunits to adhere to specific ECM coatings. For example, β

Indexed as

CorneaExtracellular MatrixFibroblastsFocal AdhesionsCell AdhesionCell LineCollagenExtracellular Matrix ProteinsFibronectinsHumansIntegrinsCollagenExtracellular Matrix ProteinsFibronectinsIntegrinscollagen fibrilscorneacorneal fibroblastsfocal adhesionsintegrinsmicropatternstopography

Identifiers

PMID41465361
PMCPMC12733271

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.