Evidence map›Paper›PMID 41465336›Full record

ArticleInternational journal of molecular sciences2025

Structural and Proteomic Analysis of the Mouse Cathepsin B-DARPin 4m3 Complex Reveals Species-Specific Binding Determinants.

Miki Zarić, Livija Tušar, Lovro Kramer, Olga Vasiljeva, Matej Novak, Francis Impens, Aleksandra Usenik, Kris Gevaert, Dušan Turk, Boris Turk

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Miki ZarićMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0009-0008-1364-5197
Livija TušarMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0000-0003-0423-3963
Lovro KramerMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.
Olga VasiljevaMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0000-0003-3648-5460
Matej NovakMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.
Francis ImpensVIB-UGent Center for Medical Biotechnology, UGent Department of Biomolecular Medicine, 9052 Ghent, Belgium.ORCID 0000-0003-2886-9616
Aleksandra UsenikMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0000-0003-2366-2886
Kris GevaertVIB-UGent Center for Medical Biotechnology, UGent Department of Biomolecular Medicine, 9052 Ghent, Belgium.ORCID 0000-0002-4237-0283
Dušan TurkMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0000-0003-0205-6609
Boris TurkMolecular and Structural Biology, Department of Biochemistry, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia.ORCID 0000-0002-9007-5764

Funding

The Slovenian Research and Innovation Agency P1-0140; P1-0048
6 · The paper itself

Abstract

Cathepsin B (CatB) is a lysosomal cysteine protease that plays a major role in various pathologies and is therefore considered a valuable therapeutic target. To address species-specific inhibitor challenges, we characterized the selective binding of designed ankyrin repeat protein (DARPin) 4m3 toward mouse cathepsin B (mCatB) over human CatB (hCatB). The mCatB-DARPin 4m3 complex was validated by size-exclusion chromatography (SEC), nano-differential scanning fluorimetry (nano-DSF), and surface plasmon resonance (SPR), revealing high affinity binding (K

Indexed as

Cathepsin BProteomicsAnimalsBinding SitesHumansMiceProtein BindingSpecies SpecificitySurface Plasmon ResonanceCathepsin BCtsb protein, mousecathepsincrystal structureDARPinprotein inhibitorproteomics

Identifiers

PMID41465336
PMCPMC12732864

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.