ReviewInternational journal of molecular sciences2025
Analysis of qPCR Data: From PCR Efficiency to Absolute Target Quantity.
Review in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Limit of Blank Variability as a Determinant of Analytical Sensitivity in a DNA-Binding Dye-Based Digital Polymerase Chain Reaction for the Detection of Chlamydia pneumoniae.Analytical chemistry · 2026Article
- In Vitro Anti-Breast Cancer Effects ofInternational journal of molecular sciences · 2026Article
- [Guideline development for MRD diagnostics in AML as a blueprint for solid tumors].Pathologie (Heidelberg, Germany) · 2026Review
- Implementation and Validation of a Limiting Component Quantification Method for qPCR.International journal of molecular sciences · 2026Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative Polymerase Chain Reaction (qPCR) is a very sensitive method to determine small amounts of DNA or RNA in experimental, environmental, veterinary, forensic and clinical samples. Despite efforts from the qPCR community to address qPCR variability by recommending standardization of reporting of all steps of a qPCR experiment, most reported qPCR results are still grossly biased. The first part of this paper describes two decades of efforts to remedy this situation by promoting so-called efficiency-corrected qPCR data analysis. Although such analysis leads to less variable qPCR results, the outcome, fluorescence at cycle zero, is difficult to grasp. In the second part, we outline how qPCR analyses can result in N
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Registered trials
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