Integrating WGCNA, TCN, and Alternative Splicing to Map Early Caste Programs in Day-2 Honeybee Larvae.

Xiang Ding, Jinyou Li, Dan Yue, Runlang Su

Abstract read

In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

ACmix-Swin Deep Learning of 4-Day-OldGenes · 2025
Article

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5 · Who and what money

Authors and funding

4 authors.

Xiang DingUniversity of Chinese Academy of Sciences, Beijing 100049, China.

Jinyou LiFaculty of Computing & Data Sciences, Boston University, Boston, MA 02215, USA.

Dan YueCollege of Animal Science, Yunnan Agricultural University, Kunming 650201, China.ORCID 0000-0002-2646-4386

Runlang SuCollege of Animal Science, Yunnan Agricultural University, Kunming 650201, China.ORCID 0009-0004-5028-4782

Funding

Yiwu Industrial and Commercial College Program under Grant Nos XJKJ2502YBYiwu Industrial and Commercial University XJKJ2502YB

6 · The paper itself

Abstract

BACKGROUND/

objectivesThe larval stage plays a pivotal role in determining caste and sex in

methodsRNA-seq was conducted on 2-day-old larvae from all three castes. Differential expression, WGCNA, and alternative splicing patterns were investigated. A deep learning TCN model was trained using WGCNA-derived modules and demonstrated high classification accuracy.

resultsThe TCN model highlighted a top-10 gene set, including

conclusionsThe 2-day-old larval stage is crucial for both caste and sex differentiation in honeybees. This study identifies key genes and splicing events, offering new insights into the molecular mechanisms underlying caste formation and sex determination.

Indexed as

Alternative SplicingGene Regulatory NetworksInsect ProteinsAnimalsBeesFemaleGene Expression Regulation, DevelopmentalLarvaMaleSex DifferentiationTranscriptomeInsect Proteinsalternative splicingApis melliferacastedeep learningWGCNA

Identifiers

PMID41465082

DOI10.3390/genes16121409

PMCPMC12733025

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.