A Brief Review on the Analysis of dsDNA, RNA, Amino Acids and Bacteria by Capillary Electrophoresis.

Yuan Zeng, Ping Wang, Bo Yang, Yuqing Xu, Yueqing Wang, Zhenqing Li, Yoshinori Yamaguchi

Abstract readReview

In one paragraph

Review in Bioengineering (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuan ZengCollege of Medical Imaging, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.

Ping WangDepartment of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China.

Bo YangEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.

Yuqing XuEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.

Yueqing WangEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.

Zhenqing LiEngineering Research Center of Optical Instrument and System, Ministry of Education, Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, No. 516 JunGong Road, Shanghai 200093, China.ORCID 0000-0001-9060-7305

Yoshinori YamaguchiComprehensive Research Organization, Waseda University, Tokyo 162-0041, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Capillary electrophoresis (CE) is an effective tool for the analysis of many biocomponents, such as dsDNA, RNA, amino acids and bacteria, which are extremely important not only in research work but also in numerous practical applications. However, there are many factors that affect the separation performance, including the polymers inside the capillary, the electric field strength, the capillary coating and the effective length of the capillary. So far, various CE techniques have been developed to increase the resolution, sample volume consumption and limit of detection. To better understand the development of techniques for the separation of these biomolecules by CE, this review provides a comprehensive summary of polymers (e.g., polyvinylpyrrolidone, hydroxyethyl cellulose and polyethylene glycol), optimization methods, capillary coating methods, technological advancement of microchips for CE and the limitation of detection proposed by different groups worldwide. We also discuss the challenges and future directions associated with CE technology.

Indexed as

amino acidbacteriacapillary coatingcapillary electrophoresisdsDNAmicrochip capillary electrophoresisRNA

Identifiers

PMID41463603

DOI10.3390/bioengineering12121306

PMCPMC12729857

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.