Spatial Heterogeneity and Methodological Insights in Fish Community Assessment: A Case Study in Hulun Lake.

Zifang Liu, Yuetong Zhang, Yanan Pan, Zhousunxi Ma, Xin Han, Ziqi Zhou, Shuang Tian, Bingjiao Sun

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In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Zifang LiuGuangdong Provincial Key Laboratory of Water Quality Improvement and Ecological Restoration for Watersheds, School of Ecology, Environment and Resources, Guangdong University of Technology, Guangzhou 510006, China.ORCID 0009-0003-4088-1797

Yuetong ZhangChina National Environmental Monitoring Centre, Beijing 100012, China.

Yanan PanCollege of Fisheries Sciences, Tianjin Agricultural University, Tianjin 300392, China.

Zhousunxi MaCollege of Fisheries Sciences, Tianjin Agricultural University, Tianjin 300392, China.

Xin HanChina National Environmental Monitoring Centre, Beijing 100012, China.

Ziqi ZhouChina National Environmental Monitoring Centre, Beijing 100012, China.

Shuang TianChina National Environmental Monitoring Centre, Beijing 100012, China.

Bingjiao SunChina National Environmental Monitoring Centre, Beijing 100012, China.

Funding

Department of Science and Technology of Inner Mongolia Autonomous Region 2024ZD13

6 · The paper itself

Abstract

Hulun Lake, a UNESCO Biosphere Reserve, faces mounting threats from extreme climate events and anthropogenic pressures, highlighting the need for robust biodiversity monitoring. Environmental DNA (eDNA) has emerged as a promising tool for aquatic biomonitoring, yet different bioinformatic pipelines-such as Amplicon Sequence Variant (ASV) and Operational Taxonomic Unit (OTU) clustering-may yield divergent results. This study compares ASV and OTU clustering approaches in eDNA metabarcoding alongside traditional capture-based surveys to assess fish diversity in Hulun Lake. Across all methods, we identified 43 taxa (40 species), including the critically endangered

Indexed as

ASV vs. OTUbiodiversity monitoringfish community structurespatial ecology

Identifiers

PMID41463451

DOI10.3390/biology14121678

PMCPMC12730304

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.