Evidence map›Paper›PMID 41463437›Full record

ArticleBiology2025

Construction and Performance Evaluation of an Astaxanthin-Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells.

Jiang Wu, Siqi Liu, Xiaowen Zeng, Yang Li, Yinlin Yao, Jing Wang, Guangdong Hu, Kai Kang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jiang WuCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0000-0002-0771-1791
Siqi LiuCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Xiaowen ZengCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Yang LiCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.ORCID 0009-0006-4353-8814
Yinlin YaoCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
Jing WangCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
Guangdong HuCollege of Animal Science and Technology, Shihezi University, Shihezi 832000, China.ORCID 0009-0003-8486-4227
Kai KangCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Funding

the Marine Biology Foundation of Zhanjiang Bureau of Science and Technology 2021E05028the Natural Science Foundation of Guangdong Province 2020A1515110362the Science and Technology Program of XPCC 2024DA006the Undergraduate Innovation Project of the College of Coastal Agricultural Sciences 202501
6 · The paper itself

Abstract

Spermatogonial stem cells (SSCs) are the only adult male germline stem cells capable of lifelong self-renewal and differentiation into spermatozoa. Scalable ex vivo survival is essential for endangered species germplasm banking, genetic resource conservation, and male infertility therapy. Here, chitosan (CO) or chitosan oligosaccharide (COS) was cross-linked into injectable, biodegradable 3D hydrogels loaded with the natural astaxanthin (AST). CCK-8 optimization identified 0.3% CO + 0.2% AST (CHAG) and 0.2% COS + 0.2% AST (COAG) as superior formulations. After 7 or 14 d of 3D culture, CHAG yielded significantly more colonies than controls (

Indexed as

3D hydrogelastaxanthinchitosan/chitosan oligosaccharidespermatogonial stem cellstranscriptomics

Identifiers

PMID41463437
PMCPMC12729764

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.