Evaluation of Human Dental Pulp Stem Cells Expressing BMP2/7 Heterodimers in a Doxycycline-Inducible Manner.

Edit Hrubi, Ferenc Tóth, Gergely Nagy, József Tőzsér, Csaba Hegedűs

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Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Edit HrubiDepartment of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, H-4032 Debrecen, Hungary.

Ferenc TóthDepartment of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, H-4032 Debrecen, Hungary.ORCID 0000-0002-1170-6274

Gergely NagyDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary.

József TőzsérDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032 Debrecen, Hungary.ORCID 0000-0001-5076-8729

Csaba HegedűsDepartment of Biomaterials and Prosthetic Dentistry, Faculty of Dentistry, University of Debrecen, H-4032 Debrecen, Hungary.ORCID 0000-0003-4143-2507

Funding

Ministry of Culture and Innovation of Hungary from the National Research TKP2021-EGA-20

6 · The paper itself

Abstract

The BMP2/7 heterodimer is known as a stronger inducer of osteogenic differentiation than BMP2 or BMP7 homodimers. Our aim was to establish BMP2/7-expressing human dental pulp stem cells (DPSCs) to evaluate tReceived: 23 October 2025; Revised: 29 November 2025; Accepted: 3 December 2025; Published: 3 December 2025 he osteogenic potential of the genetically modified cells. Lentiviral transduction was used to introduce the Tet-ON-regulated transgene-containing vector to the cells. Endogenous heterodimers were detected at the mRNA and protein levels using RNA-seq, qPCR, and Western blot, while secreted heterodimers were detected using ELISA assays. Osteogenic differentiation was monitored by measuring alkaline phosphatase (ALP) activity, mineralization, and the expression levels of

Indexed as

Bone Morphogenetic Protein 2Bone Morphogenetic Protein 7Dental PulpDoxycyclineStem CellsAlkaline PhosphataseCell DifferentiationCells, CulturedHumansOsteogenesisProtein MultimerizationAlkaline PhosphataseBMP2 protein, humanBMP7 protein, humanBone Morphogenetic Protein 2Bone Morphogenetic Protein 7DoxycyclineBMP2/7bone regenerationDPSCgene therapyosteogenic differentiationTet-ON

Identifiers

PMID41463360

DOI10.3390/biom15121704

PMCPMC12730885

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.