Production of biodiesel and biolubricant via co-cultivation of Arthrospira platensis and Rhodosporidium babjevae using pulp-paper mill sludge hydrolysate.

Hossein Fazelian, Mostafa Keshavarz Moraveji, Mehrdad Mozaffarian, Farzaneh Vahabzadeh

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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4 authors.

Hossein FazelianDepartment of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran.

Mostafa Keshavarz MoravejiDepartment of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran. moraveji@aut.ac.ir.

Mehrdad MozaffarianDepartment of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran.

Farzaneh VahabzadehDepartment of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The pulp and paper manufacturing industry generates large volumes of sludge, which is characterized by a high COD content known as pulp and paper mill sludge (PPMS). Co-cultivation of Arthrospira platensis and Rhodosporidium babjevae in the present study was evaluated quantitatively by the production of high-value products through lipid biosynthesis by using PPMS hydrolysate. We pretreated PPMS in two steps: dilute-acid hydrolysis followed by enzymatic hydrolysis. Dilute-acid hydrolysis was statistically optimized using a central composite design, yielding optimum conditions of 1.03% (w/w) nitric-sulfuric acid, 134.8 °C, and 23.6 min. Subsequently, the PPMS hydrolysate underwent enzymatic saccharification for 72 h at 50 °C with 2% (w/w) commercial cellulase. The co-cultivation process thereafter was also optimized using Box-Behnken design, where affecting parameters at optimized levels were 3.86 klux light intensity, 1.48 L min

Indexed as

BasidiomycotaBiofuelsPaperSewageSpirulinaCoculture TechniquesHydrolysisIndustrial WasteBiofuelsIndustrial WasteSewageBiodiesel-biolubricant productionLipid biosynthesisPPMS hydrolysateResponse surface methodologyRhodosporidium babjevae–Arthrospira platensis co-cultivationWastewater bioremediation

Identifiers

PMID41462058

DOI10.1038/s41598-025-28597-z

PMCPMC12748702

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.