Evidence map›Paper›PMID 41461875›Full record

ArticleScientific reports2025

Rapid virus-free production of recombinant yellow fever virus envelope protein and its in-depth biophysical analysis.

Luca Schelle, Wolfgang Kuttenlochner, Victoria Sanchez, Barbara Steigenberger, Katja Finkl, Marius Schmid, Celine Douat, Einar Halldorsson, Maren Schubert, Sabine Suppmann

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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Luca Schelle *Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Immunology, Infection and Pandemic Research IIP, Nonnenwald 2, D-82377, Penzberg, Germany.
Wolfgang Kuttenlochner *Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Immunology, Infection and Pandemic Research IIP, Nonnenwald 2, D-82377, Penzberg, Germany.
Victoria SanchezMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152, Martinsried, Germany.
Barbara SteigenbergerMax Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152, Martinsried, Germany.
Katja FinklFraunhofer Institute for Translational Medicine and Pharmacology ITMP, Immunology, Infection and Pandemic Research IIP, Nonnenwald 2, D-82377, Penzberg, Germany.
Marius SchmidZentriForce Pharma Research GmbH, Carl-Friedrich-Gauß-Ring 5, Überherrn, Heidelberg, D-69124, Germany.
Celine DouatInstitute for Chemical Epigenetics, Ludwig-Maximilians-Universität, Butenandtstraße 5-13, D-81377, Munich, Germany.
Einar HalldorssonFIDA Biosystems Aps, Generatorvej 6 A + B, Søborg, 2860, Denmark.
Maren SchubertHelmholtz Centre for Infection Research, Inhoffenstraße 7, D-38124, Braunschweig, Germany.
Sabine SuppmannFraunhofer Institute for Translational Medicine and Pharmacology ITMP, Immunology, Infection and Pandemic Research IIP, Nonnenwald 2, D-82377, Penzberg, Germany. Sabine.Suppmann@itmp.fraunhofer.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to develop a rapid and easy-to-adapt protocol for recombinant production of Orthoflavivirus proteins. We introduced the new-generation pOpiE2 vector for virus-free transient transfection of Trichopluisa ni High Five insect cells to produce the envelope protein ectodomain of Yellow Fever Virus Asibi and 17D strains. We achieved high yields in the range of 6–7 mg/L within five working days. In-depth biophysical analysis demonstrated highest protein purity, homogeneity, stability, and affinities to specific antibodies in the low nanomolar range. A combination of peptide mass fingerprint and intact mass spectrometry characterized the copurified precursor chaperone peptide pr and discovered a new posttranslational processing site for the recombinantly produced protein.

Indexed as

Recombinant ProteinsViral Envelope ProteinsYellow fever virusAnimalsCell LineGenetic VectorsRecombinant ProteinsViral Envelope Proteins

Identifiers

PMID41461875
PMCPMC12753717

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.