Evidence map›Paper›PMID 41461775›Full record

ArticleScientific reports2025

The kinases Hsl101 and Urk1 regulate metabolism of Cryptococcus neoformans.

Yuanyuan Ma, Yetong Shen, Lei Zhang, Wenjie Fang, Weihua Pan, Wanqing Liao, Xin Ye, Xinyao Liu, Yanyan Xu, Xiaojing Li and 2 more

Abstract read
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In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Yuanyuan Ma *Nantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, China. myycsd@ntu.edu.cn.
Yetong Shen *College of Life and Health Sciences, Northeastern University, Shenyang, China.
Lei Zhang *Department of Dermatology, Shaanxi Provincial People's Hospital, The Third Affiliated Hospital of Xi'an Jiaotong University, Xian, China.
Wenjie FangDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Weihua PanDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Wanqing LiaoDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China.
Xin YeDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xian, China.
Xinyao LiuLaboratory of Pathogen Research of West China Hospital, Sichuan University, Chengdu, China.
Yanyan XuAffiliated Hospital of Hebei University of Engineering, Handan, Hebei, China.
Xiaojing LiSchool of Clinical Medicine, Hebei University of Engineering, Handan, China. zlmdsh@126.com.
Peng XueNantong Key Laboratory of Environmental Toxicology, Department of Occupational Medicine and Environmental Toxicology, School of Public Health, Nantong University, Nantong, China. pengxue@ntu.edu.cn.
Liuyang CaiDepartment of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital, Naval Medical University, Shanghai, 200003, China. liuyang_cai@126.com.

Funding

Key Project of Shaanxi Science and Technology Department 2024SF2-GJHX-02Ministry of Science and Technology 2022YFC2504803Nantong Jiangsu Scientific Research Project of China JC2023043National Natural Science Foundation of China 82302546National Natural Science Foundation of China 82472304Natural Science Foundation of Jiangsu Province of China BK20240948Natural Science Foundation of Sichuan Province 2023NSFSC1550Natural Science Research of Jiangsu Higher Education Institutions of China 24KJD430010Shanghai Science and Technology Committee 20DZ2272000
6 · The paper itself

Abstract

Cryptococcus neoformans is a significant human fungal pathogen, particularly concerning for immunocompromised individuals. Key kinases, Hsl101 and Urk1, are critical for crossing the blood-brain barrier, although the specific mechanisms by which they do so remain undefined. In this study, we systematically investigate the impact of HSL101 or URK1 deletion on the proteomic and metabolomic profiles of C. neoformans. By generating a deletion mutant for HSL101, we observed profound alterations in the expression levels of 366 proteins and 421 metabolites, highlighting significant disruptions in key biological processes, such as oxidative phosphorylation and ATP synthesis, which are vital for energy production in the cell. Similarly, the deletion of URK1 resulted in significant changes in the expression of 236 proteins and 366 metabolites, particularly affecting pathways related to steroid biosynthesis and starch and sucrose metabolism, which are critical for cellular function and adaptation. These findings shed light on the regulatory roles of Hsl101 and Urk1 in the metabolic pathways of C. neoformans.

Indexed as

Cryptococcus neoformansFungal ProteinsProtein KinasesGene DeletionGene Expression Regulation, FungalHumansMetabolomicsProteomicsFungal ProteinsProtein KinasesCryptococcus neoformansHsl101KinaseMetabolomicsProteomeUrk1

Identifiers

PMID41461775
PMCPMC12749253

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