Evidence map›Paper›PMID 41461768›Full record

ArticleScientific reports2025

Isolation, structure revision and stereochemistry of trichomycins A and B, aromatic analogues of amphotericin B.

Tomasz Laskowski, Filip Anaszewicz, Julia Borzyszkowska-Bukowska, Julia Pakuła, Małgorzata Michałowska, Julia Bublewska, Dorota Gudanis-Sobocińska, Katarzyna Kozłowska-Tylingo, Paweł Szczeblewski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tomasz LaskowskiDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Filip AnaszewiczDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Julia Borzyszkowska-BukowskaDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Julia PakułaDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Małgorzata MichałowskaDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Julia BublewskaDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Dorota Gudanis-SobocińskaDepartment of Biomolecular NMR, Institute of Bioorganic Chemistry Polish Academy of Sciences, Zygmunta Noskowskiego Str. 12/14, Poznań, 61-704, Poland.
Katarzyna Kozłowska-TylingoDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland.
Paweł SzczeblewskiDepartment of Pharmaceutical Technology and Biochemistry and BioTechMed Centre, Faculty of Chemistry, Gdansk University of Technology, Gabriela Narutowicza Str. 11/12, Gdańsk, 80-233, Poland. pawel.szczeblewski@pg.edu.pl.

Funding

Politechnika Gdańska DEC-47/2021/IDUB/I.3.3
6 · The paper itself

Abstract

Fungal infections pose an increasing global health threat, particularly to immunocompromised individuals. In response, the World Health Organization has prioritized the development of new antifungal agents. Among clinically used polyene macrolides, trichomycin remains structurally ambiguous despite decades since its discovery. This study presents an integrated re-assessment of the trichomycin complex focused on its two principal constituents using a combination of liquid chromatography-mass spectrometry (HPLC-DAD-ESI-MS), preparative-scale HPLC, two-dimensional NMR spectroscopy, and computational modeling. Four principal components were identified, including trichomycins A and B and their respective all-trans isomers, formed via light-induced isomerization. One-step prep-HPLC isolation enabled the purification of both trichomycins in analytically pure form. Detailed NMR analysis redefined the polyol fragment (C1-C14) of trichomycin A and confirmed structural consistency in trichomycin B. Absolute configurations of 13 stereocenters in each molecule were assigned by integrating experimental ³J coupling constants, ROE data, and molecular modeling, incorporating the generalized Karplus equation. The chromophore geometry was determined as 22E, 24E, 26E, 28Z, 30Z, 32E, 34E. By resolving key structural inconsistencies, this work lays the foundation for the rational derivatization of trichomycin and supports future efforts toward the development of novel polyene-based antifungal agents with improved pharmacological profiles.

Indexed as

Amphotericin BAntifungal AgentsChromatography, High Pressure LiquidMagnetic Resonance SpectroscopyModels, MolecularMolecular StructureSpectrometry, Mass, Electrospray IonizationStereoisomerismAmphotericin BAntifungal Agents

Identifiers

PMID41461768
PMCPMC12748807

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.