Evidence map›Paper›PMID 41461717›Full record

SynthesisScientific reports2025

Effects of microalgae supplementation on performance, egg quality, yolk fatty acid levels, and blood parameters in laying hens: a meta-analysis.

Moh Sofi'ul Anam, Uswatun Muslykhah, Muhammad Maulana Sadid, Cuk Tri Noviandi, Andriyani Astuti, Dimas Hand Vidya Paradhipta, Ali Agus

Abstract readMeta-Analysis
In one paragraph

Synthesis in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Moh Sofi'ul AnamDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia. m.sofiul.a@mail.ugm.ac.id.
Uswatun MuslykhahTropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
Muhammad Maulana SadidDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Cuk Tri NoviandiDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Andriyani AstutiDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Dimas Hand Vidya ParadhiptaDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Ali AgusDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microalgae have gained attention in laying hen nutrition due to their richness in n-3 polyunsaturated fatty acids (PUFA), pigments, and antioxidants. However, their efficacy in poultry diets remains inconsistent. This meta-analysis quantitatively evaluated the effects of dietary microalgae supplementation on performance, egg quality, yolk fatty acid composition, and blood biochemistry in laying hens. Thirty-six peer-reviewed articles were retrieved from Scopus, ScienceDirect, PubMed, and Google Scholar following PRISMA guidelines, and effect sizes were calculated using weighted mean differences. Microalgae supplementation did not affect feed intake (p > 0.05) but significantly improved hen-day egg production, egg weight, egg mass, yolk weight, shell traits, and Haugh units (p < 0.05). Yolk pigmentation increased in color intensity and redness (p < 0.001) but decreased in lightness (p < 0.001). Microalgae enhanced yolk total n-3 PUFA, eicosapentaenoic acid, and docosahexaenoic acid (p < 0.001), while reducing n-6 PUFA and the n-6/n-3 ratio (p < 0.05). Blood total cholesterol, aspartate aminotransferase, and alanine aminotransferase concentrations decreased (p < 0.05). In conclusion, dietary microalgae supplementation augments laying performance, enhances egg quality and yolk pigmentation, fortifies yolk with beneficial n-3 fatty acids, and promotes liver health, highlighting its potential as a functional feed additive in layer diets.

Indexed as

ChickensDietary SupplementsEggsEgg YolkFatty AcidsMicroalgaeAnimal FeedAnimalsFatty Acids, Omega-3FemaleFatty AcidsFatty Acids, Omega-3Egg qualityLaying hensMicroalgaeN-3 polyunsaturated fatty acidsPerformance

Identifiers

PMID41461717
PMCPMC12748610

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.