Evidence map›Paper›PMID 41461685›Full record

ArticleScientific reports2025

Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.

Zongzhe Xuan, Qiuyue Peng, Mariia Borsuk, Rupali Prasad, Vladimir Zachar, Suman Kumar Das, Cristian Pablo Pennisi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Nanozyme-based therapies for inflammatory immune disorders: opportunities and challenges.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
    Review
  2. Review
  3. Integrative Adipogenic Engineering of Cultured Fat for Cell Based Meat.Comprehensive reviews in food science and food safety · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zongzhe XuanRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Selma Lagerløfs vej 249, Gistrup, 9260, Denmark.
Qiuyue PengRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Selma Lagerløfs vej 249, Gistrup, 9260, Denmark.
Mariia BorsukMirai International AG, Herdernstrasse 71, Zurich, 8004, Switzerland.
Rupali PrasadMirai International AG, Herdernstrasse 71, Zurich, 8004, Switzerland.
Vladimir ZacharRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Selma Lagerløfs vej 249, Gistrup, 9260, Denmark.
Suman Kumar DasMirai International AG, Herdernstrasse 71, Zurich, 8004, Switzerland.
Cristian Pablo PennisiRegenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Selma Lagerløfs vej 249, Gistrup, 9260, Denmark. cpennisi@hst.aau.dk.

Funding

Eurostars E! 115596
6 · The paper itself

Abstract

Cultivated meat provides a sustainable and ethical alternative to traditional livestock production, addressing global challenges in meat demand and environmental impact. Fat is recognized as an essential component of meat products, but strategies for culturing animal fat precursors still have major limitations, such as the need for a serum-containing medium and inefficient adipogenic cell maturation. In this study, a serum-free medium (SFM) formulation for the cultivation of bovine adipose-derived stem cells (bASC) was first validated by comparison with serum-containing media (FBS). After a 14-day induction period, the SFM increased bASC proliferation by 20% and triglyceride accumulation by 66%. When the SFM was applied to a three-dimensional (3D) spheroid culture system, it resulted in up to 34% higher triglyceride accumulation compared to 2D cultures. These results were accompanied by a significant increase in the expression of key adipogenic markers and lipid droplet formation, suggesting a synergistic potential of SFM and 3D spheroid strategies for bASC culture. Overall, the results of this study provide a scalable and sustainable platform for cultured fat production and are an important step towards overcoming some of the challenges in cultured meat production.

Indexed as

AdipocytesAdipose TissueCell Culture TechniquesCell Culture Techniques, Three DimensionalStem CellsAdipogenesisAnimalsCattleCell DifferentiationCell ProliferationCells, CulturedCulture Media, Serum-FreeSpheroids, CellularTriglyceridesCulture Media, Serum-FreeTriglycerides3D spheroid cultureAdipogenic differentiationBovine adipose stem cellsCultured fatSerum-free mediumSustainable meat production

Identifiers

PMID41461685
PMCPMC12749105

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.