ArticleCurrent microbiology2025
Exploring the Dynamic Culture System of biofilm-forming Bifidobacterium Adolescentis BL-8: from System establishment, Formation Mechanisms Analysis To Application in freeze-dried Probiotic Powder.
Article in Current microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Biofilm formation provides probiotics with a natural barrier against harsh conditions and increases their bioavailability. The dynamic culture system could effectively promote probiotic biofilm formation. In view of the excellent probiotic properties and biofilm-forming ability of Bifidobacterium adolescentis BL-8, a dynamic culture system for this strain was constructed in this study and utilized to carry out in-depth mechanistic and potential application research. The results showed that innovatively establishing this system with physical-biological synergies for B. adolescentis BL-8, using oat bran as the carrier, achieved a 46.6% increase in the biofilm-forming rate and reduced the formation time by 12 h. This enhancement was attributed to carrier-strain interactions that this system strengthened bacterial initial aggregation during reversible adhesion through electrostatic/hydrophilic force regulation. Transcriptome analysis during irreversible adhesion and growth maturation phase further discovered that this system controls two-component signaling systems to boost nitrogen assimilation and c-di-GMP pathways to suppress flagella assembly, collectively promoting bacterial growth and morphological transitions. Dynamic cultivation also regulated the quorum-sensing system, prolonged biofilm growth, and stimulated extracellular polymeric substance synthesis. These modulations reinforced the biofilm's structural integrity, enabling increased bacterial resistance to freeze-drying and gastrointestinal stress and the storage time of the freeze-dried probiotic powder. This dynamic culture system with physical-biological synergies effectively promoted B. adolescentis BL-8 biofilm formation through enhanced initial adhesion, bacterial proliferation and morphological transformations, and prolonged biofilm formation, exhibiting great potential for applications. This study provides an important theoretical basis and new insights for the development of probiotic biofilm-modulation technology and its industrial applications.
Indexed as
Identifiers
41460350What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.