Evidence map›Paper›PMID 41460065›Full record

ArticleAngewandte Chemie (International ed. in English)2026

A Nonviral Neo-Nucleocapsid for Cell-Specific RNA Delivery Developed by Pseudo-Cyclic Peptide Grafting and Directed Evolution.

Daiki Kanayama, Naohiro Terasaka, Kazuki Kato, Sae Okazaki, Hiroaki Suga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daiki KanayamaDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Naohiro TerasakaDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.ORCID 0000-0002-4988-6899
Kazuki KatoStructural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo, 153-8904, Japan.ORCID 0000-0003-1141-0059
Sae OkazakiStructural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo, 153-8904, Japan.
Hiroaki SugaDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.ORCID 0000-0002-5298-9186

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
Advanced Research Infrastructure for Materials and Nanotechnology in Japan (ARIM) JPMXP1223UT0022Astellas Foundation for Research on Metabolic DisordersJapan Science and Technology Agency JPMJAP24B3Japan Science and Technology Agency JPMJFR2310Japan Science and Technology Agency JPMJPR21EAJapan Society for the Promotion of Science JP20H05618Japan Society for the Promotion of Science JP22KJ0865NIGMS NIH HHS R01 GM129325The International Graduate Program for Excellence in Earth-Space Science
6 · The paper itself

Abstract

Nonviral protein vehicles for RNA delivery hold significant promises for various biotechnological applications due to their scalability, ease of engineering, and high biocompatibility. Directed evolution is a powerful approach to enhance protein activity, yet few nonviral protein vehicles have been evolved due to the challenges in linking genotype and phenotype. Here, we report engineering and directed evolution of a neo-nucleocapsid enabling receptor-specific RNA delivery. We applied the DogTag/DogCatcher protein ligation system to an artificial nucleocapsid and conducted directed evolution to obtain nucleocapsid displaying proteins. To create designer neo-nucleocapsids, we lasso-grafted a pharmacophore sequence of a pseudo-cyclic peptide binding to the hepatocyte growth factor receptor MET into the loop of DogCatcher. After the cellular-based directed evolution, endocytic uptake of neo-nucleocapsids was improved whereas cytosolic release and subsequent protein expression from the delivered RNA were not detected. This experimental approach provides a versatile system for constructing protein cages delivering various cargos to specific cells.

Indexed as

Directed Molecular EvolutionPeptides, CyclicRNAHumansPeptides, CyclicRNADirected evolutionDrug deliveryPeptidesProtein engineeringReceptors

Identifiers

PMID41460065
PMCPMC12887611

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.