Impact of oxidative stress on

Magnetotactic bacteria, such as IMPORTANCE: Magnetotactic bacteria (MTB) are fascinating microorganisms with the capacity to produce iron oxide nanomagnets, namely magnetosomes. These nanomagnets passively align with magnetic fields, providing the basis for magnetic guidance when coupled with bacterial motility. This unique feature has inspired the concept of MTB as a self-propelled medical device to deliver therapeutic cargo. To this end, survival in physiological conditions and other microenvironments is essential. This study reveals the interplay between MTB physiology and magnetosome biomineralization subject to oxidative stress and varying environmental conditions, employing a holistic multi-parametric approach. Our results provide an in-depth understanding of the metabolic and physiological mechanisms at the single-cell level. This is crucial to develop more robust MTB biomanufacturing strategies to produce bacteria that possess the required quality attributes, thus paving the way for future biotechnological and biomedical application studies.