Evidence map›Paper›PMID 41459724›Full record

ArticleJournal of molecular cell biology2026

Screening the composition of tubulin isotypes reveals the most abundant TUBB4B for ciliary polarity in ependymal cells.

Yanling Liu, Mingyi Liu, Huaqing Xue, Boran Chang, Fengxia Shao, Suhao Cao, Jinsong Li, Xu Zhang, Lei Diao, Lan Bao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yanling LiuKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Mingyi LiuSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Huaqing XueKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Boran ChangKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Fengxia ShaoKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Suhao CaoKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Jinsong LiKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.ORCID 0000-0003-3456-662X
Xu ZhangSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Lei DiaoKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Lan BaoKey Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Funding

CAMS 2019-I2M-5-082National Natural Science Foundation of China 31991194
6 · The paper itself

Abstract

Cilia are microtubule-based organelles that protrude from the surface of various eukaryotic cell types. Microtubules are assembled by α/β-tubulin heterodimers, all of which comprise multiple isotypes encoded by distinct genes. However, the composition and function of tubulin isotypes in cilia are largely unclear. Here, we successfully labeled the endogenous α-tubulin and β-tubulin isotypes with HA or GFP tag in cultured mouse ependymal cells (EPCs) via the CRISPR/Cas9 system. TUBA1A, TUBA1B, TUBA1C, TUBB2A, TUBB2B, TUBB4B, and TUBB5 were identified to be incorporated in ependymal cilia, with TUBB4B showing the highest expression. Overexpression assay revealed that the ependymal cilia did not display a preference for the entrance of specific tubulin isotypes. Furthermore, luciferase reporter assay showed that the expression of TUBB4B in EPCs was specifically regulated by the ciliogenesis factor FOXJ1. TUBB4B deficiency disrupted planar polarity of EPCs and impaired cerebrospinal fluid flow, resulting in hydrocephalus. This study reveals the composition of tubulin isotypes in ependymal cilia and the specific role of FOXJ1-promoted TUBB4B in ciliary motility.

Indexed as

Cell PolarityCiliaEpendymaTubulinAnimalsCells, CulturedForkhead Transcription FactorsHydrocephalusMiceProtein IsoformsForkhead Transcription FactorsFOXJ1 protein, mouseProtein IsoformsTubulinependymal cellsmotile ciliaTUBB4Btubulin isotype

Identifiers

PMID41459724
PMCPMC13389950

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.