ArticleFrontiers in plant science2025
Multiplex CRISPR/Cas9-mediated editing of seven glycosyltransferase homologs in
Article in Frontiers in plant science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- AI-Assisted Spatial Metabolic Engineering in Plants: Integrating Flux Design, Spatial Omics, and Synthetic Biology.Metabolites · 2026Review
- Editorial: Plant molecular farming for biopharmaceutical production and beyond.Frontiers in plant science · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
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Abstract
Introduction: Plant-based systems hold great potential for producing therapeutic proteins, but differences in N-glycosylation pathways between plants and mammals present major technical and regulatory barriers. In particular, plant-specific α-1,3-fucosylation and β-1,2-xylosylation can generate immunogenic glycan structures, necessitating genome engineering to humanize plant glycosylation profiles. Methods: We applied multiplex CRISPR/Cas9 genome editing in Nicotiana benthamiana to simultaneously target five α-1,3-fucosyltransferase genes and two β-1,2-xylosyltransferase genes. Resulting T Results: Two T Discussion: This work establishes the first
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Registered trials
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