ArticleJOR spine2025
A Multiscale Ex Vivo Method to Investigate Intervertebral Disc Strain and Fiber Recruitment in Anterolateral Bending Using 9.4T MRI-DVC and DIC Microscopy.
Article in JOR spine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
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5 authors.
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Abstract
Study Design: Ex vivo, multiscale analysis of disc strain using ultrahigh-field MRI-based digital volume correlation (MRI-DVC) and differential interference contrast (DIC) microscopy. Objective: To evaluate the relationship between three-dimensional strain distributions and collagen fiber recruitment in porcine cervical intervertebral discs under flexion and lateral bending. Summary of Background Data: Flexion combined with lateral bending is often linked to disc herniation, yet the strain patterns and fiber-level changes in the annulus fibrosus are not well understood. Multiscale characterization is essential to uncovering failure mechanisms. Methods: Four porcine cervical motion segments were scanned in neutral and anterolaterally (AL)-bent postures using 9.4T MRI, with 3D strains calculated via DVC. Samples were sectioned and imaged with DIC microscopy to quantify collagen fiber recruitment based on fiber crimp patterns, using a crimp grading scale (0 = fully straight, 1 = semi-crimped, 2 = uncrimped). Results: MRI-DVC revealed an inhomogeneous strain distribution in AL-bent discs, with higher magnitudes compared to the neutral discs. Fiber uncrimping was greater in the AL-bent discs (mean crimp grade: 0.44, mostly straight) compared with the neutral discs (1.56, predominantly crimped). Across the bending axis, the anterior-right region exhibited higher strains than the posterior-left (minimum principal strain ~25% greater), which correlated with the presence of sequential lamellae having straight and fully-crimped fibers. A greater amount of fiber uncrimping was observed in the posterior-left than anterior-right disc regions. Conclusion: This study confirms the suitability of MRI-DVC combined with DIC microscopy for relating macroscopic strains to microscopic fiber crimp, and for identifying regions of high strain across multiple length scales. Under AL-bending, this methodology revealed that the disc's posterior region exhibited taut fibers, which may contribute to its susceptibility to herniation.
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