ArticleFrontiers in microbiology2025
Isolation, identification, and genomic analysis of bacteriophage GJKY-A for the prevention and treatment of citrus canker.
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- The gp38 protein inhibits host adsorption of phage vB_EcoM_SD286.Frontiers in microbiology · 2026Article
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9 authors.
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Abstract
Introduction: Citrus canker, caused by Methods: This study isolated bacteriophages from wastewater using a double-layer plate method, and conducted biological characterization and genomic analysis on the isolated bacteriophages. Results: We report the isolation and characterization of a phage, GJKY-A, from sewage effluent targeting Xcc. Transmission electron microscopy revealed an polyhedral head (~90 nm) and a long, flexible non-contractile tail (~150 nm). One-step growth analysis showed a latent period of 20 min and a burst size of ~84 plaque-forming units per cell. GJKY-A displayed high host specificity and remained stable across 40-50 °C and pH 3-12. Genomic sequencing identified a 62,551 bp double-stranded DNA genome with 44.81% GC content. Analysis of the taxonomic status of the phage shows that phage GJKY-A is closely related to phages of the genus Discussion: These findings establish GJKY-A as a promising biocontrol candidate and provide a foundation for developing safe, eco-friendly strategies for citrus canker management.
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