Evidence map›Paper›PMID 41458290›Full record

ArticleReproductive medicine and biology

Development, Live Birth and Fertility in Mouse Oocytes Penetrated by Spermatids Exposed to the HDAC Inhibitor Scriptaid.

Seiji Watanabe, Youichi Takemoto, Rei Inoue, Izumi Tanaka, Hironori Miyata, Yuya Makino, Motoi Nagayoshi, Daisuke Nakajima, Shigeki Fujimoto, Atsushi Tanaka

Abstract read
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In one paragraph

Article in Reproductive medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Seiji WatanabeDepartment of Anatomical Science Hirosaki University Graduate School of Medicine Hirosaki Japan.
Youichi TakemotoDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Rei InoueDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Izumi TanakaDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Hironori MiyataAnimal Research Center, Faculty of Medicine University of Occupational and Environmental Health Kitakyushu Japan.
Yuya MakinoDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.ORCID https://orcid.org/0009-0000-3428-3166
Motoi NagayoshiDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Daisuke NakajimaDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Shigeki FujimotoDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.
Atsushi TanakaDepartment of Obstetrics and Gynecology Saint Mother Clinic Kitakyushu Japan.ORCID https://orcid.org/0000-0001-5299-2505

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: In mouse embryos derived from round spermatid injection (ROSI), low developmental potential due to abnormal histone modification is improved by histone deacetylase (HDAC) inhibitors. However, some studies suggest that the use of such inhibitors leads to the birth of hypomorphic offspring. We examined the genetic risk of Scriptaid on mouse ROSI embryos over two generations. Methods: Oocytes were penetrated by spermatids treated with Scriptaid (250 nM 10 min) and transferred to host mothers. After the offspring were born and matured, some first-generation males and females were mated to obtain second-generation offspring. One-cell ROSI embryos treated with Scriptaid (250 nM 16 h) underwent the same process. Results: Scriptaid reduced H3K9me3 residues in the spermatid nuclei and in the male pronuclei of ROSI embryos, resulting in increased blastocyst formation and live birth rates compared with the untreated group. All offspring exposed to Scriptaid had normal body weight and external appearance and subsequently grew as well as the IVF offspring. Additionally, all second-generation offspring were also healthy. Conclusion: Scriptaid improved the developmental potential of ROSI embryos, and no clear teratogenic effect was observed in the live offspring, suggesting that Scriptaid is safer than Trichostatin-A, which has been reported to induce hypomorphic offspring in ROSI.

Indexed as

epigeneticsHDACROSIround spermatidteratogenicity

Identifiers

PMID41458290
PMCPMC12740194

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.