Evidence map›Paper›PMID 41458150›Full record

ArticleTransboundary and emerging diseases2025

Molecular Characterization and Pathogenicity of a Novel Recombinant NADC30-Like PRRSV Strain Isolated From Piglets Inducing Intestinal Inflammation, and Construction of Its Infectious Clone.

Yafang Lin, Yan Ouyang, Jiayang Zheng, Changguang Xiao, Yang Yang, Qianming Zhao, Yan Zhang, Zongjie Li, Ke Liu, Beibei Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yafang LinKey Laboratory of Animal Microbiology of China's Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China, njau.edu.cn.ORCID https://orcid.org/0009-0000-8448-0491
Yan OuyangCollege of Agriculture, Hubei Three Gorges Polytechnic, Yichang, 443000, China.ORCID https://orcid.org/0009-0007-8848-5362
Jiayang ZhengShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Changguang XiaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Yang YangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Qianming ZhaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Yan ZhangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.ORCID https://orcid.org/0000-0003-2216-5749
Zongjie LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Ke LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Beibei LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Donghua ShaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Yafeng QiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.
Zhiyong MaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.ORCID https://orcid.org/0000-0002-6723-0113
Jianchao WeiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China, caas.cn.ORCID https://orcid.org/0000-0002-3246-6273

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in swine production worldwide, characterized by reproductive failure and respiratory disease. In China, the cocirculation of diverse strains drives ongoing viral evolution and the emergence of novel recombinants, posing serious challenges for disease control. Here, we report the isolation and characterization of a novel PRRSV strain (JS-YZ/2023) from a PRRS-suspected pig farm. Genomic analysis revealed a unique recombinant pattern between NADC30-like and HP-PRRSV lineages, distinct from previously reported recombinants. Experimental infection in piglets demonstrated significant pathogenicity, with persistent fever, severe anorexia, progressive weight loss, and pathological lesions including interstitial pneumonia with lymphocyte infiltration and marked disruption of intestinal villi. Notably, JS-YZ/2023 exhibited strong intestinal tropism, inducing a local cytokine storm in the small intestine and showing PRRSV-N protein antigen accumulation in crypt epithelial cells, as detected by immunohistochemistry. In addition, we successfully constructed a full-length infectious cDNA clone of JS-YZ/2023 incorporating a green fluorescent protein (GFP) reporter gene (rGFP-JS-YZ/2023). This engineered virus provides a valuable tool for investigating the pathogenic mechanisms of NADC30-like recombinants and facilitating vaccine development.

Indexed as

Porcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusAnimalsChinaInflammationPhylogenySwineVirulenceinfectious cloneintestinal tropismNADC30-like PRRSVpathogenicityrecombination analysisviral isolation

Identifiers

PMID41458150
PMCPMC12741902

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.