ArticleClinical and experimental dental research2025
The Effect of Pellicle on Biofilm Formation in a Supragingival Biofilm Model.
Article in Clinical and experimental dental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Quorum Sensing and Quorum Quenching in Periodontal Disease: Mechanisms and Therapeutic Potential.Current issues in molecular biology · 2026Review
- Salivary proteins, proteases, and disease pathophysiology in oral health and precision diagnostics: a narrative review.Frontiers in dental medicine · 2026Review
- The Effect of Pellicle on Biofilm Formation in a Supragingival Biofilm Model.Clinical and experimental dental research · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
objectivesOral biofilms initiate with the formation of an acquired pellicle on dental surfaces, a thin layer of salivary glycoproteins that provides a substrate for microbial adhesion. This study aimed to assess the necessity of a preformed pellicle for biofilm growth in vitro by analyzing the development of a standardized six-species biofilm, comprising Actinomyces oris, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, Streptococcus oralis, and Candida albicans. MATERIALS AND
methodsBiofilms were cultivated on bovine enamel discs under two conditions: (1) precoated with human saliva to simulate a pellicle and (2) without a preformed pellicle. Colony-forming units (CFUs) of each microbial species were quantified after incubation in either human saliva or a NaCl-based medium at 16 and 64 h.
resultsThe analysis revealed no significant differences in CFU counts between discs with or without a preformed pellicle, regardless of whether biofilms were grown in human saliva or NaCl medium, with one exception: S. oralis in pellicle/NaCl (6.7 Log
conclusionsThese findings suggest that microbial adhesion and subsequent biofilm development occurred independently of an initial pellicle. The preformed salivary pellicle does not seem to play a significant role in the initial development of this in vitro biofilm model. Biofilm testing in laboratory settings, especially for studies on antimicrobial efficacy, could be simplified, as pellicle formation may not be an essential requirement. Although no significant differences in biofilm development were observed between pellicle and no-pellicle conditions, the growth medium may have influenced pellicle interactions, warranting further investigation of media effects on pellicle formation. Existing assumptions about pellicle dependence in biofilm formation are challenged, and suggest that in vitro models without a pellicle may still provide valid platforms for studying biofilms and testing antimicrobial agents effectively.
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Registered trials
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