ArticleACS synthetic biology2026
Resource Competition and Growth Dilution Modulate Synthetic Gene Cascade Dynamics.
Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Investigating synergistic effects of key medium components and pectin elicitor on taxane fermentation in Neopestalotiopsis vitis: A Box-Behnken response surface methodology approach.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Coupled Perturbations of Gene Circuit Dynamics by Resource Competition and Growth Dilution.Methods in molecular biology (Clifton, N.J.) · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
In synthetic biology, a key goal is to design robust and stable genetic circuits with accurate and predictable behavior. Modularity is a central principle in circuit design, enabling the construction of complex systems from smaller, well-characterized parts. However, resource competition presents a major obstacle to modularity by disrupting gene expression dynamics. Here, we constructed and characterized a library of inhibitory genetic cascades with varied promoter strengths, RBS strengths, and plasmid backbones. We found that increasing the expression of the downstream module could unexpectedly lead to a reduction in the expression of the upstream regulatory module by competing for shared cellular resources. These results indicate that resource limitations can transform a unidirectional inhibitory cascade into an unintended feedback loop. In addition, we found that growth-mediated dilution can reshape gene expression patterns, further influencing circuit dynamics. Together, these findings underscore the critical roles of both resource competition and growth dilution in shaping the behavior of synthetic gene circuits.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.