Evidence map›Paper›PMID 41457305›Full record

ArticleCNS neuroscience & therapeutics2025

High Shear Stress-Induced Endothelial Piezo1 Downregulation Promotes Intracranial Aneurysm Formation via the PDGF-BB/PDGFRβ Paracrine Signaling Pathway.

Zhiwen Lu, Sisi Li, Fengfeng Xu, Haishuang Tang, Shijie Zhu, Chuanchuan Wang, Xiaohua Yang, Qinghai Huang

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PubMed Full text at PMC Publisher
In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Zhiwen LuDepartment of Neurosurgery, Naval Medical Center, The PLA Naval Medical University, Shanghai, China.
Sisi LiDepartment of Neurovascular Intervention, Clinical Center of Neuroscience, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.ORCID 0000-0002-0533-0469
Fengfeng XuDepartment of Neurosurgery, Naval Medical Center, The PLA Naval Medical University, Shanghai, China.
Haishuang TangDepartment of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, China.ORCID 0000-0003-0520-4187
Shijie ZhuDepartment of Neurovascular Centre, Changhai Hospital, Naval Medical University, Shanghai, China.ORCID 0000-0002-2297-7571
Chuanchuan WangDepartment of Neurovascular Centre, Changhai Hospital, Naval Medical University, Shanghai, China.
Xiaohua YangCentral Laboratory, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Qinghai HuangDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.ORCID 0000-0002-5612-5190

Funding

National Natural Science Foundation of China 82071279National Natural Science Foundation of China 82371314
6 · The paper itself

Abstract

backgroundAbnormally high shear stress (HSS) is strongly associated with intracranial aneurysm (IA) formation. Endothelial Piezo1 is sensitive to shear stress stimulation, but the mechanism by which it mediates this mechanobiological coupling process is unclear.

methodsThe correlation between shear stress and the Piezo1 expression was investigated using human IA samples and a parallel-plate flow chamber system. To determine the effects of endothelial Piezo1 on the phenotype of neighboring vascular smooth muscle cells (VSMCs) and IA formation, the CRISPR/Cas9 system was used to inhibit endothelial Piezo1 gene expression in vitro. Piezo1

resultPiezo1 expression correlated negatively with the shear stress in human IA. HSS reduced EC Piezo1 expression and promoted VSMC phenotypic transformation compared with physiological shear stress. Depletion of EC Piezo1 resulted in the VSMC phenotypic transformation and, more importantly, promoted aneurysmal vascular remodeling in the mouse IA model. The platelet-derived growth factor subunit B (PDGFB)_Platelet-derived growth factor receptor β (PDGFRβ) was identified as being involved in this process. Moreover, the PDGFRβ antagonist reversed the VSMC phenotypic transformation and attenuated IA progression. Mechanistically, Piezo1 depletion promoted PDGFB expression via YAP/β-catenin pathway.

conclusionHSS downregulates Piezo1 expression in ECs, which subsequently enhances PDGF-BB expression through the YAP/β-catenin signaling pathway. The elevated PDGF-BB facilitates phenotypic transition of VSMCs via PDGFRβ binding, ultimately contributing to IA formation.

Indexed as

BecaplerminEndothelial CellsIntracranial AneurysmIon ChannelsParacrine CommunicationReceptor, Platelet-Derived Growth Factor betaAdultAgedAnimalsCerebral ArteriesCoculture TechniquesComputer SimulationDisease Models, AnimalDown-RegulationFemaleHemodynamicsBecaplerminIon ChannelsPIEZO1 protein, humanPiezo1 protein, mouseReceptor, Platelet-Derived Growth Factor betaintracranial aneurysmphenotype transformationPiezo1platelet‐derived growth factor subunit BB (PDGF‐BB)shear stress

Identifiers

PMID41457305
PMCPMC12745340

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.