Evidence map›Paper›PMID 41456912›Full record

ArticleMolecular plant pathology2025

Virus-Inducible Activation of Hypersensitive Response Confers Broad-Spectrum Resistance to Diverse Viruses in Plants.

Ran-Ran Pan, Ting Xie, Ya-Jie Wang, Hong-Da Song, Wilmer J Cuellar, Yan Xie, Shu-Sheng Liu, Xiao-Wei Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular plant pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ran-Ran PanState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Ting XieState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Ya-Jie WangNational Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, China.
Hong-Da SongState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Wilmer J CuellarVirology Laboratory, Cassava Program, International Center for Tropical Agriculture (CIAT), Palmira, Colombia.
Yan XieState Key Laboratory of Rice Biology and Breeding, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Shu-Sheng LiuState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Xiao-Wei WangState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.ORCID 0000-0002-7472-3944

Funding

National Key Research and Development Program 2022YFC2601000National Natural Science Foundation of China 32161143008
6 · The paper itself

Abstract

The hypersensitive response (HR) is a powerful plant defence mechanism that restricts pathogen spread through localised cell death. However, constitutive activation of HR can impair growth and development, limiting its utility in crop breeding and protection. In the study, we developed a virus-inducible HR system by coupling an Avirulence/Resistance (Avr/R) gene pair, Avr4/Cf-4 effector-resistance gene pair, with a promoter (Solyc04g076730) specifically activated by geminivirus infection. We demonstrate that co-expression of Avr4 and Cf-4 in Nicotiana benthamiana triggers robust HR and significantly reduces accumulation of multiple geminiviruses, including TYLCV, TYLCCNV, SLCMV and BSCTV. Transcriptomic analysis of virus-infected tomato identified Solyc04g076730 as a virus-responsive promoter with minimal basal activity. Transient assays and stable transformation in both N. benthamiana and tomato revealed that this promoter effectively drives HR only upon viral infection, resulting in reduced viral loads, attenuated symptoms and improved plant vigour. Importantly, transgenic plants expressing the Solyc04g076730::Avr4/Cf-4 construct maintained normal growth under non-infected conditions. Our findings offer a promising strategy for engineering broad-spectrum resistance to viral pathogens in crops.

Indexed as

Disease ResistanceGeminiviridaeNicotianaPlant DiseasesSolanum lycopersicumGene Expression Regulation, PlantPlant ProteinsPlants, Genetically ModifiedPromoter Regions, GeneticPlant Proteinsgeminivirushypersensitive responseinducible promoterR genevirus resistance

Identifiers

PMID41456912
PMCPMC12744956

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.