Evidence map›Paper›PMID 41455472›Full record

ArticleStem cell reports2026

Chaperonin proteins CCT5 and CCT7 epigenetically restrict the transition from pluripotency to totipotency in embryonic stem cells.

Jiali Jiang, Zhiyong Liu, Xiaye Miao, Shilong Han, Min Li, Linxi Xie, Hejiao Zhang, Fuwen Zuo, Qingsheng Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiali JiangDepartment of Laboratory Medicine, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China; School of Basic Medical Science, Anhui Medical University, Hefei, Anhui, China.
Zhiyong LiuDepartment of Pharmacology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
Xiaye MiaoDepartment of Laboratory Medicine, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China.
Shilong HanDepartment of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Min LiDepartment of Laboratory Medicine, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China.
Linxi XieSchool of Basic Medical Science, Anhui Medical University, Hefei, Anhui, China.
Hejiao ZhangDepartment of Gastroenterology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Fuwen ZuoDepartment of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic address: fuwenzuo@email.sdu.edu.cn.
Qingsheng HanDepartment of Laboratory Medicine, Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China; Department of Pharmacology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China. Electronic address: 201915113@mail.sdu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As members of the CCT family, CCT5 and CCT7 play pivotal roles in telomerase trafficking, with their depletion resulting in TCAB1 protein loss and impaired telomere maintenance. However, their functional significance in embryonic stem cells (ESCs) state transitions remains incompletely understood. Here, we demonstrate that CCT5 or CCT7 deficiency disrupts telomere length homeostasis, triggering DNA damage response pathways and inducing epigenetic reprogramming. This cascade enhances cellular plasticity, activates repeat elements and 2-cell transcriptional programs, and facilitates the generation of 2-cell-like cells, suggesting that CCT5 and CCT7 may serve as epigenetic barriers restricting the transition from pluripotency to totipotency. Additionally, CCT5/7 stabilizes pluripotency through Wnt/β-catenin signaling: CCT7 directly binds β-catenin to facilitate nuclear translocation, while CCT5 dissociates E-cadherin/β-catenin complexes. These findings underscore the dual role of CCT5 and CCT7 in maintaining telomere integrity and regulating pluripotent state dynamics.

Indexed as

Embryonic Stem CellsEpigenesis, GeneticMouse Embryonic Stem CellsPluripotent Stem CellsAnimalsbeta CateninCadherinsCell DifferentiationCellular ReprogrammingHumansMiceProtein BindingTelomereTelomere HomeostasisWnt Signaling Pathwaybeta CateninCadherins2-cell-like cellsCCT5/7cell fate determinationembryonic stem cellheterochromatin

Identifiers

PMID41455472
PMCPMC12925953

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.