ArticleLangmuir : the ACS journal of surfaces and colloids2026
An Optimized Positive Staining Protocol Method for Clear Visualization of Extracellular Vesicles by TEM Using Uranyless and Lead Citrate.
Article in Langmuir : the ACS journal of surfaces and colloids, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- SEM mapping of sequence-specific protein-DNA interactions on long DNA molecules.Nucleic acids research · 2026Article
- Dysregulation of the HSF1-Mediated UPRBiomolecules · 2026Article
- Ultracentrifugation and Ultrafiltration Differentially Alter the Composition and Functionality of the Biomolecular Corona of Extracellular Vesicles.Journal of extracellular biology · 2026Article
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7 authors.
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Abstract
Transmission electron microscopy (TEM) is widely used for visualizing extracellular vesicles (EVs) due to its nanometer-scale resolution. However, existing sample preparation protocols often lack sufficient methodological detail, requiring researchers to rely on trial-and-error approaches to achieve high-quality images. Here, we report an optimized positive-staining method that utilizes the sequential application of Uranyless and lead citrate with an intermediate buffered wash. This approach yields high image contrast, preserves EV morphology, and significantly reduces background artifacts. Staining specificity and elemental localization were confirmed using Energy-Dispersive X-ray Spectroscopy (EDS). The protocol is reproducible and robust, offering a reliable framework for EV structural analysis and potentially serving as a reference standard for TEM-based EV imaging.
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