Evidence map›Paper›PMID 41454414›Full record

ArticleStem cell research & therapy2025

Hypoxic TCs-preconditioned MSCs ameliorate acute lung injury via enhanced Treg recruitment and function through CXCL5/6-CXCR1 axis.

Luoyue Yin, Xu Zhang, Yile Zhou, Huihui Ju, Youwei Zhu, Rongrong Gao, Pinwen Wu, Hao Fang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Luoyue Yin *Department of Anaesthesiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Xu Zhang *NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai, 200237, China.
Yile Zhou *Department of Anaesthesiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Huihui JuDepartment of Anaesthesiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Youwei ZhuClinical Center of Bio-Therapy at Zhongshan Hospital & Institutes of Biomedical Sciences, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Rongrong GaoClinical Center for Biotherapy at Zhongshan Hospital, Fudan University, Shanghai, 200032, China. lsq274241075@163.com.
Pinwen WuDepartment of Anesthesiology, Minhang Hospital, Fudan University, Shanghai, China. wpw_1976@163.com.
Hao FangDepartment of Anaesthesiology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. drfanghao@163.com.

Funding

Minhang District natural science research topic 2024MHZ101National Natural Science Foundation of China 82272230Natural Science Foundation of Shanghai, China 22ZR1444700
6 · The paper itself

Abstract

backgroundAcute lung injury/acute respiratory distress syndrome (ALI/ARDS) remains a critical respiratory condition with limited effective treatments.

methodsThis study investigated whether mesenchymal stem cells (MSCs) preconditioned with supernatant from hypoxia-cultured telocytes (TCs) could enhance therapeutic efficacy in ALI through regulatory T cell (Treg) modulation.

resultsMSCs preconditioned with 5% hypoxic TC supernatant demonstrated superior efficacy in ameliorating LPS-induced lung injury compared to conventional MSCs or TC monotherapy, as evidenced by preserved alveolar architecture, reduced inflammatory infiltration, and decreased pro-inflammatory cytokines. Mechanistically, these preconditioned MSCs significantly enhanced Treg recruitment to injured lung tissues and improved their immunosuppressive function through the CXCL5/6-CXCR1 axis, an effect that was substantially attenuated upon siRNA-mediated disruption of this pathway, and was further corroborated in a humanized ALI mouse model where preconditioned-MSC treatment improved survival, reduced lung injury severity, and enhanced Treg recruitment and function in a CXCL5/6 signaling-dependent manner.

conclusionsThese findings reveal a novel mechanism by which hypoxic TC supernatant enhances MSC therapeutic efficacy in ALI through the CXCL5/6-CXCR1 axis, providing a promising strategy for optimizing cellular therapy in inflammatory pulmonary disorders.

Indexed as

Acute Lung InjuryChemokine CXCL5Chemokine CXCL6Mesenchymal Stem CellsMesenchymal Stem Cell TransplantationReceptors, Interleukin-8AT-Lymphocytes, RegulatoryAnimalsCell HypoxiaDisease Models, AnimalHumansLipopolysaccharidesMaleMiceMice, Inbred C57BLSignal TransductionChemokine CXCL5Chemokine CXCL6LipopolysaccharidesReceptors, Interleukin-8AAcute lung injuryMesenchymal stem cellsRegulatory T cellsTelocytes

Identifiers

PMID41454414
PMCPMC12849632

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.