ArticleBMC veterinary research2025
Identification and characterization of a novel linear B-cell epitope on nucleocapsid protein of porcine epidemic diarrhea virus.
Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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10 authors.
Funding
Abstract
backgroundPorcine epidemic diarrhea virus (PEDV) is a highly virulent enterovirus that is responsible for severe diarrhea in neonatal piglets. For no effective vaccine or specific treatment are available, rapid and accurate diagnosis is crucial for the prevention and control of disease. The nucleocapsid (N) protein is conserved with strong immunogenicity, making it an important target for developing serological approaches.
resultsIn this study, soluble PEDV N protein was expressed, purified, and then used to produce monoclonal antibodies (mAbs). Three mAbs, named 8G2, 6F3, and 3E1, against PEDV N protein were generated. These mAbs were identified as IgG1 isotypes with κ light chains, exhibited high specificity for PEDV, and showed no cross-reactivity with transmissible gastroenteritis virus (TGEV) or porcine deltacoronavirus (PDCoV). Epitope mapping revealed 8G2 specifically recognized a novel linear B-cell epitope at 274-DLKDIPEWR-282 of viral N protein by using a serious of truncated overlapping peptides. Homology analysis showed that the epitope of 8G2 was highly conserved (100%) among different PEDV strains. Structural assay suggested that the identified epitope was located on the surface of PEDV N protein. Functional analysis demonstrated 8G2 could be used in indirect immunofluorescence assay, western blot, flow cytometry, and immunohistochemistry.
conclusionsThese mAbs help us better understanding the antigenicity of PEDV N protein, and serve as robust tools for serological diagnosis and vaccine development.
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