ArticleApplied microscopy2025
Correlative x-ray microscopy and transmission electron microscopy for ultrastructural analysis of zebrafish tissue.
Article in Applied microscopy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We established a correlative workflow combining X-ray microscopy (XRM) and transmission electron microscopy (TEM) to investigate the ultrastructure of zebrafish neuromasts. Zebrafish tissues were processed using conventional TEM embedding protocols and first imaged with XRM to obtain three-dimensional information and to localize the neuromast within resin blocks. The XRM previews enabled accurate trimming and rapid identification of target regions, reducing the need for repeated semi-thin sectioning and toluidine blue staining. Subsequent TEM analysis revealed ultrastructural details including cell-to-cell contacts and organelle morphology. Our study demonstrates that integration of XRM with TEM increases efficiency, minimizes tissue loss, and improves orientation accuracy for ultrastructural analysis. This correlative approach provides a valuable workflow for volume electron microscopy, applicable to the observation of the neuromast and various other tissues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.