ArticleStem cell reviews and reports2026
Exploring a Flow Cytometry-Based CFU Assay for Functional Assessment of Human HSPCs: a Robust Alternative to Morphological Colony Evaluation.
Article in Stem cell reviews and reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
6 authors.
Funding
Abstract
backgroundAssessment of hematopoietic stem and progenitor cells (HSPC) function is essential for both research and clinical applications. Traditional colony-forming unit (CFU) assays, based on morphological evaluation in semi-solid media, are labor-intensive and subject to operator bias, limiting reproducibility and standardization.
methodsWe evaluated a commercially available, flow cytometry-based liquid CFU assay for quantifying HSPC proliferation and differentiation. Using a high-throughput platform, colonies were identified and classified by the expression of CD14, CD15, and CD235a (Glycophorin A). Results were compared with conventional semi-solid CFU assays.
resultsThe flow-based assay demonstrated strong correlation with traditional methods, while reducing both hands-on time and inter-operator variability. The approach allowed objective, reproducible classification of BFU-E, CFU-G, CFU-GM, CFU-M, and CFU-GEMM colonies, and was compatible with clinically relevant sample types.
conclusionThe liquid CFU assay provides a standardized, efficient, and robust alternative for functional evaluation of human HSPCs, supporting its broad adoption in both research and clinical settings.
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