Evidence map›Paper›PMID 41452382›Full record

ArticlePlant cell reports2025

Genome-wide analysis of Zoysia japonica REVEILLE family identifies ZjRVE8-1 as a positive cold-tolerance regulator.

Cuiling Liu, Liangliang He, Ming Jiang, Zhihao Wu, Jieyi Li, Chao Liu, Quanrui Su, Jingyi Sun, Fei Xu, Shu Chen

Abstract read
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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Cuiling LiuDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Liangliang HeDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Ming JiangDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Zhihao WuDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Jieyi LiDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Chao LiuDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Quanrui SuDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Jingyi SunDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Fei XuDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.
Shu ChenDepartment of Grass Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. shuchen@scau.edu.cn.ORCID http://orcid.org/0000-0001-8122-4193

Funding

Guangdong Basic and Applied Basic Research Foundation 2023A1515140005Guangdong Basic and Applied Basic Research Foundation 2025A1515010836Guangzhou Municipal Science and Technology Projec 2024E04J1233Zhongshan Mingcai Intelligent Lighting Technology Co., LTD. 2023ZSCXZX02
6 · The paper itself

Abstract

key messageFunctional analysis in Arabidopsis revealed that ZjRVE8 -1 significantly enhances cold tolerance by activating the CBF pathway, identifying it as a promising gene for breeding cold hardy Zoysia japonica cultivars. REVEILLE (RVE) transcription factors regulate circadian rhythms and abiotic stress responses, but their roles in cold tolerance in the warm-season turfgrass Zoysia japonica remain uncharacterized. We identified 10 RVE family members through genome-wide analysis and performed comprehensive expression profiling under multiple abiotic stresses, revealing cold-responsive patterns. Based on this, five cold-induced RVE genes were selected for functional validation via heterologous overexpression in Arabidopsis thaliana. Overexpression of ZjRVE8-1 significantly enhanced freezing tolerance under both non-acclimated (NA) and cold-acclimated (CA) conditions by upregulating CBF1 and CBF3 expression. In contrast, overexpression of four tested RVE1-clade members failed to improve, and in one case reduced, freezing tolerance, correlating with their divergent cold-induced expression patterns. Promoter analysis identified a low-temperature responsive (LTR) element exclusively in ZjRVE8-1 of the cold-tolerant genotype, explaining its superior induction. This study uncovers ZjRVE8-1 as a novel regulator of cold adaptation via CBF activation and provides functional insights into RVE family divergence, offering candidate genes for breeding cold-hardy Z. japonica.

Indexed as

Plant ProteinsPoaceaeTranscription FactorsArabidopsisCold-Shock ResponseCold TemperatureGene Expression Regulation, PlantGenome, PlantGenome-Wide Association StudyPlants, Genetically ModifiedPromoter Regions, GeneticPlant ProteinsTranscription FactorsCBF pathwayCold toleranceFunctional divergencePromoter polymorphismREVEILLE gene familyZoysia japonica

Identifiers

PMID41452382

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.