Evidence map›Paper›PMID 41452270›Full record

ArticleACS applied bio materials2026

Zinc-Mediated Loading and Release of His-Tagged Recombinant Proteins in Self-Assembling Peptide Coacervates.

Benjamin Clegg, Gayathri Aparnasai Reddy, Ketki Y Velankar, Sarah M Ostrowski, Wen Liu, Yong Fan, Ellen S Gawalt, Wilson S Meng

Abstract read
In one paragraph

Article in ACS applied bio materials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Benjamin CleggDepartment of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, United States.
Gayathri Aparnasai ReddyGraduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, United States.ORCID 0000-0001-8210-6359
Ketki Y VelankarGraduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, United States.
Sarah M OstrowskiDepartment of Pharmacy and Therapeutics, School of Pharmacy, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.
Wen LiuAllegheny Health Network Cancer Institute, Allegheny Health Network, Pittsburgh, Pennsylvania 15212, United States.
Yong FanAllegheny Health Network Cancer Institute, Allegheny Health Network, Pittsburgh, Pennsylvania 15212, United States.
Ellen S GawaltDepartment of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, United States.
Wilson S MengGraduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, United States.ORCID 0000-0003-2575-2260

Funding

Novel hematopoietic humanized mouse model to study CAR-T therapy-associated cytokine release syndromeR21OD034476 · OD · ALLEGHENY-SINGER RESEARCH INSTITUTE · PI FAN, YONG · 2023 to 2024
$445k
Injectable 3D-Clustered Human Fibroblastic Reticular Cells for Expanding Regulatory T CellsR21AI185527 · NIAID · DUQUESNE UNIVERSITY · PI YONG FAN, Wilson S Meng · 2025 to 2026
$415k
NIAID NIH HHS R21 AI185527NIH HHS R21 OD034476
6 · The paper itself

Abstract

The development of tunable systems for subcutaneous injection is currently the focus of exploratory protein formulation. For the delivery of protein biologics intended for extended release, a high fraction of the drug that escapes from the deposition site ("burst release") may pose safety concerns. Herein, we report an injectable system of bioaffinity zinc-containing peptide coacervates in which recombinant proteins coexpressed with histidine (His)-tags can be captured and released over time. Coacervates are formed by driving self-assembling peptide (SAP), AEAEAKAKAEAEAKAKHHHHHH (EAKH6) β-sheet dimers, into cross-linking fibrils. In the presence of Zn

Indexed as

Biocompatible MaterialsHistidinePeptidesRecombinant ProteinsZincAnimalsMaterials TestingMiceParticle SizeBiocompatible MaterialsHistidinePeptidesRecombinant ProteinsZincbioaffinitycoordination bondlong-acting injectableself-assembling peptidesustained release

Identifiers

PMID41452270
PMCPMC12820974

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.