Integrated 3D-Printed Microfluidic Device for Immunocapture and Electrochemical Assessment of Transferrin Saturation in

Davide Paolini, Silvia Dortez, Marta Pacheco, Teresa Gasull, Dario Compagnone, Flavio Della Pelle, Alberto Escarpa

Abstract read

In one paragraph

Article in ACS sensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Davide PaoliniDepartment of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, 28802 Alcala de Henares, Madrid, Spain.

Silvia DortezDepartment of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, 28802 Alcala de Henares, Madrid, Spain.

Marta PachecoDepartment of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, 28802 Alcala de Henares, Madrid, Spain.

Teresa GasullCellular and Molecular Neurobiology Research Group, Department of Neurosciences, Germans Trias i Pujol Research Institute (IGTP), 08916 Badalona, Barcelona, Spain.ORCID 0000-0002-9321-1741

Dario CompagnoneDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100 Teramo, Italy.ORCID 0000-0001-7849-8943

Flavio Della PelleDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100 Teramo, Italy.ORCID 0000-0002-8877-7580

Alberto EscarpaDepartment of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcala, 28802 Alcala de Henares, Madrid, Spain.ORCID 0000-0002-7302-0948

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A 3D-printed electrochemical microfluidic device (3D-EMD) was developed to assess the transferrin saturation (TSAT) biomarker in ischemic stroke patients. The all-in-one 3D-EMD integrates a strategically engineered immunoassay module for the direct and selective isolation of transferrin (Tf) from unpretreated clinical samples, unaffected by sample coloration, with an interchangeable electrochemical sensor for the simultaneous detection of Tf and Tf-bound iron. Both modules are interconnected through microfluidic channels whose flow is regulated by a cylindrical rotary valve. The analytical workflow enables magnetic bead-based direct Tf immunocapture and simultaneous electrochemical detection of Tf and Tf-bound iron via square wave voltammetry, allowing TSAT assessment within 60 min using only 50 μL of sample. Validation with certified reference materials demonstrated excellent accuracy (

Indexed as

Electrochemical TechniquesLab-On-A-Chip DevicesPoint-of-Care SystemsPrinting, Three-DimensionalStrokeTransferrinBiomarkersHumansImmunoassayIronPoint-of-Care TestingBiomarkersIronTransferrin3D-sensorfused deposition modelingsquare wave voltammetrytransferrintransferrin-bound iron

Identifiers

PMID41451703

DOI10.1021/acssensors.5c02834

PMCPMC12836336

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.