ArticleFrontiers in veterinary science2025
Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Innovations, Applications, and Future Trends in Veterinary Diagnostic Technologies.Transboundary and emerging diseases · 2026Review
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Authors and funding
8 authors.
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Abstract
Introduction: The African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques. The World Organization of Animal Health (WOAH) has recommended two highly sensitive and specific real time polymerase chain reaction (PCR) procedures (P1 qPCR and P2 qPCR) for the reliable and rapid detection of the ASF virus (ASFV). The present work aimed to standardize two third-generation PCR technologies by using the same WOAH-recommended primer/probe sets. Methods: We developed two droplet digital PCR protocols ddPCRs and compared their analytical performances to the above-mentioned qPCRs. This involved testing serially diluted plasmid containing the vp72 gene sequence of the ASFV as the template. Clinical positive and negative samples were also analyzed to compare both PCR procedures. Results and discussion: The ddPCR assays demonstrated excellent linearity (
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