Evidence map›Paper›PMID 41450652›Full record

ArticleJACS Au2025

Enzymology and Structural Basis of Glycosyltransferases Involved in Saponin C28 Carboxylic Acid

Graham A Hudson, Jose H Pereira, Peter H Winegar, David M FitzGerald, Andy DeGiovanni, Xiaoyue Chen, Xixi Zhao, Maria C T Astolfi, James Reed, Amr El-Demerdash and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JACS Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Graham A HudsonJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.ORCID https://orcid.org/0000-0002-3715-4279
Jose H PereiraJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Peter H WinegarJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.ORCID https://orcid.org/0000-0003-0984-4990
David M FitzGeraldJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Andy DeGiovanniJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Xiaoyue ChenJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Xixi ZhaoJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Maria C T AstolfiJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
James ReedJohn Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.
Amr El-DemerdashJohn Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.
Martin RejzekJohn Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.ORCID https://orcid.org/0000-0002-5091-544X
Shingo KikuchiJohn Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.ORCID https://orcid.org/0000-0001-9033-6242
Anne OsbournJohn Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K.ORCID https://orcid.org/0000-0003-2195-5810
Henrik V SchellerJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Paul D AdamsJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.
Jay D KeaslingJoint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California 94608, United States.ORCID https://orcid.org/0000-0003-4170-6088

Funding

User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
Synthetic biology tools for scalable production of medicinal plant terpenesR01AT010593 · NCCIH · STANFORD UNIVERSITY · PI KEASLING, JAY D, LANGE, BERND MARKUS · 2019 to 2023
$3.5M
Iterative Microbial Production and Systems Immunology to Design QS-21 AdjuvantsR01AI186111 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI JAY D KEASLING, Harshini Mukundan · 2024 to 2026
$2.6M
NCCIH NIH HHS R01 AT010593NIAID NIH HHS R01 AI186111NIGMS NIH HHS P30 GM124169Wellcome Trust 227375
6 · The paper itself

Abstract

Saponins are a class of natural products composed of an oxidized triterpene core adorned with glycosylations, ultimately giving rise to medicinally important compounds bearing bioactivity that includes, but is not limited to, anti-inflammatory, antimicrobial, antifungal, antiarrhythmic, and immunostimulatory activities. QS-21 is a prominent immunostimulatory saponin and is a critical adjuvant component of several FDA-approved vaccines. One linchpin modification in the biosynthesis and bioactivity of several saponins, including QS-21, is

Indexed as

fucosyltransferasesglycodiversificationglycosyltransferase enzymologyGT1protein structureSaponin biosynthesissaponin glycosylationX-ray crystallography

Identifiers

PMID41450652
PMCPMC12728629

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.