Evidence map›Paper›PMID 41450621›Full record

ArticleJACS Au2025

Discovery of 5‑Chlorotryptophan-Containing Antibiotics through Metabologenomics-Assisted High-Throughput Screening.

Chantal D Bader, Isao Masuda, Angela Nichols, Edward Kalkreuter, Dong Yang, Thomas Christian, Yuko Nakano, Ya-Ming Hou, Ben Shen

Abstract read
In one paragraph

Article in JACS Au, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Nicotinamide-Based Covalent Inhibitors of tRNA (mACS medicinal chemistry letters · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chantal D BaderDepartment of Chemistry, The Herbert Wertheim UF Scripps Institute for Innovation & Technology, Jupiter, Florida 33458, United States.
Isao MasudaDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States.
Angela NicholsDepartment of Chemistry, The Herbert Wertheim UF Scripps Institute for Innovation & Technology, Jupiter, Florida 33458, United States.
Edward KalkreuterDepartment of Chemistry, The Herbert Wertheim UF Scripps Institute for Innovation & Technology, Jupiter, Florida 33458, United States.
Dong YangDepartment of Chemistry, The Herbert Wertheim UF Scripps Institute for Innovation & Technology, Jupiter, Florida 33458, United States.ORCID https://orcid.org/0000-0003-2917-0663
Thomas ChristianDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States.
Yuko NakanoDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States.
Ya-Ming HouDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, United States.ORCID https://orcid.org/0000-0001-6546-2597
Ben ShenDepartment of Chemistry, The Herbert Wertheim UF Scripps Institute for Innovation & Technology, Jupiter, Florida 33458, United States.ORCID https://orcid.org/0000-0002-9750-5982

Funding

tRNA in codon usageR35GM134931 · NIGMS · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2020 to 2025
$5.4M
Discovery of Gram-negative permeable chemical probes for tRNA methylationR01AI139202 · NIAID · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2019 to 2022
$2.4M
NIAID NIH HHS R01 AI139202NIGMS NIH HHS R35 GM134931
6 · The paper itself

Abstract

Actinomycetota bacteria have specialized in the biosynthesis of antibacterial natural products (NPs), and extract and fraction libraries made from those strains remain a promising source of NP drug leads. Herein, we present a high-throughput screen (HTS), based on engineered

Indexed as

ActinomycetotaAntibioticsHigh-throughput screeningNatural ProductstRNA (guanine-N(1)−)-methyltransferase (TrmD)

Identifiers

PMID41450621
PMCPMC12728651

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.